Regulated protein degradation

Regulated protein degradation - Regulated protein...

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Regulated protein degradation - Protein degradation in the lysosomes -Endocytosis -Autophagy -Phagocytosis - Protein degradation by the proteasomes -Ubiqutin and ubiquitination (ubiquitylation) -Proteasome • Rabbit reticulocytes (without lysosomes) can degrade abnormal Hemoglobin • Develop in vitro proteolysis system • ATP dependent proteolysis • Fractionate reticulocyte system • Active principle in fraction 1 = ubiquitin • Ubiquitin is a heat stable, ubiquitous (eukaryotic cells), 76 amino acid, covalently attached to substrates Historical perspective of ubiquitination
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The structure of ubiquitin Figure 6-92a Molecular Biology of the Cell (© Garland Science 2008) Ubiquitination and deubiquitination Figure 6-92a Molecular Biology of the Cell (© Garland Science 2008) Ubiquitination Deubiquitination
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Figure 6-93 Molecular Biology of the Cell (© Garland Science 2008) Mono- and poly- ubiquitination Hicke et al. ‘05 NRMCB 6:610 Ub-I44A: a mutant that can be attached to substrates but not be recognized be
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Regulated protein degradation - Regulated protein...

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