Wang Review 1 - MCDB428 Exam 3 When: Monday, March 24th...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
MCDB428 Exam 3 When: Monday, March 24th from 6:00 p.m. - 8:00 p.m. Where: Natural Science Auditorium Format: - Multiple choices - Assays with short answers - Assays
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Materials covered - Actin cytoskeleton - Microtubules and intermediate filaments - lipid transport - Regulated protein degradation - Cell adhesion - Extracellular matrix - This review covers some major parts, but not everything of what you should know
Background image of page 2
The cytoskeleton Types Actin microfilaments Intermediate filaments Microtubules Functions Cell motility Movement of intracellular particles Vesicle trafficking Chromosome Regulation of cell shape
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Three major types of cytoskeletal fibers Microtubules: α - and β -tubulin ~ 25 nm in diameter Microfilaments: Actin, 5-9 nm in diameter; Intermediate filaments: 1. Lamins – found in nucleus 2. Keratin – epithelial cells 3. Desmin – muscle cells 4. Vimentin – mesenchymal cells ~ 10 nm in diameter
Background image of page 4
Three major types of cytoskeletal fibers Actin / substratum Tubulin / Golgi desmosome / cytokeratin / DNA
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Actin polymerization in vitro 3 phases Fast growth at (+) end ATP-bound ADP-bound Mg++, K+ or Na+ ions No nuclei added
Background image of page 6
The actin microfilaments: what do you need to know: - The unit, the assembly and the regulation (dynamic instability) - Actin binding proteins - Actin motor protein myosin (when do you need a motor when do you not?) - Rho/Rac/cdc42 GTPases in actin filaments formation - The localization and function - Muscle structure and contraction
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The Microtubules: what do you need to know: - The unit, the assembly, the regulation and dynamic instability - MT binding proteins - MT motor proteins Kinesin and dynein (orientation of movement? What do they move?) - The localization and function - MT and cell division - Cilia and flagella MTOC and MT organization
Background image of page 8
The microtubule organization interphase mitosis neuron Flagellum
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Dynamic instability of microtubules in vitro Growth rate: 1 μm/min Shrinkage: 7 μm/min Dynamic instability Rate of MT growth in vitro is slower than  shrinkage
Background image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/03/2009 for the course MCDB 428 taught by Professor Wang during the Winter '08 term at University of Michigan.

Page1 / 38

Wang Review 1 - MCDB428 Exam 3 When: Monday, March 24th...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online