recDNA_2008 - Recombinant DNA What is the basis of...

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Recombinant DNA What is the basis of recombinant DNA technology? How does one “clone” a gene? How are genetically modified organisms (GMOs) created?
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Task: Use Recombinant DNA Technology (Genetic Engineering) to Develop Gene Therapy for Cystic Fibrosis 1. Clone a functional human CFTR gene in a bacterial plasmid. 2. Create a CFTR:GFP gene fusion to track progress of recombinant CFTR in cultured epithelial cells. 3. Create a recombinant CFTR to be used in a suitable delivery vector for gene therapy in CF patients.
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Cloning a gene of interest
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Methods for obtaining a gene of interest Restriction endonuclease cleavage of genomic DNA Large fragments with introns Genomic DNA library Reverse transcription of mRNA to cDNA No introns cDNA library PCR Genomic DNA RT PCR of mRNA
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Q: An enzyme that cuts DNA at specific sequences is called: A. A DNA polymerase B. A DNA ligase C. A restriction endonuclease D. An exonuclease E. A DNase
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Q: How can DNA fragments cut with the same
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This note was uploaded on 09/04/2009 for the course BIO 1510 taught by Professor Unknown during the Spring '07 term at Georgia Institute of Technology.

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recDNA_2008 - Recombinant DNA What is the basis of...

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