Discussion 6 Fall 2008

Discussion 6 Fall 2008 - GENETICS(BIO 325 Discussion 6 Fall...

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GENETICS (BIO 325) Fall 2008 Discussion 6 _____________________________________________________________________________ _ 1. What are the three enzyme activities present in E. coli DNA polymerase I? 2. During DNA replication in E. coli , DNA was isolated at different intervals. DNA isolated at the beginning included single-stranded fragments of about 1000 nucleotides long. When DNA replication was allowed to proceed for a longer period of time, lower frequency of short fragments and many more long fragments were obtained. (a) What do the short single stranded DNA fragments correspond to (lagging strand fragments or leading strand fragment)? (b) What is the name given to these short single stranded fragments? (c) Why are long fragments observed after longer periods of synthesis? (d) What enzyme is involved in the formation of the long fragments from the short fragments? 3. During DNA replication, the lagging strand is synthesized discontinuously because (a) the lagging strand template is discontinuous. (b)
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Discussion 6 Fall 2008 - GENETICS(BIO 325 Discussion 6 Fall...

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