Discussion 7 Fall 2008 - GENETICS (BIO 325) Discussion 7 1....

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GENETICS (BIO 325) Fall 2008 Discussion 7 _____________________________________________________________________________ _ 1. The following sequence of nucleotides in a DNA strand was used as a template to synthesize an mRNA that was then translated into protein: 5’ – T-T-A-A-C-G-G-C-T-T-T-T-T-T-C- 3’ Predict the carboxy-terminal amino acid and the amino-terminal amino acid of the resulting polypeptide. Assume that the mRNA is translated starting with the first nucleotide and without the need for a start codon. 2. Transcription of gene A takes place from left to right. Transcription of gene B takes place from right to left. Which strand of DNA (top or bottom) as shown in the following figure serves as the template for transcription of each gene? 5’ 3’ 3. The following DNA sequence contains the start site of transcription of a bacterial gene. The sequences shown in bold represent the –10 (TATATT) and –35 (TTGACA) regions of the promoter. 5’-CCGTGGACCTACGTAC AATATA AGCTAGCCCTAGTCGAT TGTCAA CGGTACCGATCTAAT-3’ 3’-GGCACCTGGATGCATG TTATAT TCGATCGGGATCAGCTA ACAGTT GCCATGGCTAGATTA-5’ (a) Will the start site (+1) for RNA synthesis be to the left, right, or between the two sequences shown in bold? (b) Which of the two strands (upper or lower) will be the template strand? 4.
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Discussion 7 Fall 2008 - GENETICS (BIO 325) Discussion 7 1....

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