Unformatted text preview: Amp R gene ( Pst I). When inserted within a plasmid gene, the foreign DNA abolishes expression of the gene. Specifically, if inserted within Tet R or Amp R , sensitivity to or ampicillin would result. (d) You would select an E. coli strain carrying a "hybrid" plasmid (foreign DNA inserted into pBR322) by its pattern of sensitivity or resistance to or ampicillin. For example, if the foreign DNA were inserted into the Bam H1 site within the Tet R gene, you would select for ampicillin resistance and sensitivity. Resistance to ampicillin selects for the presence of the plasmid. Sensitivity to selects for the foreign DNA insert. 5. (a) The medium should contain ampicillin, X-gal, and nutrients. (b) Blue; the β-gal gene is functional. (c) White; the β-gal gene is disrupted by the insertion of the 1.0-kb Xba I fragment, and no β-galactosidase protein is synthesized. (d) 6. 5'-TTGCA-3' 1...
View Full Document
This note was uploaded on 09/05/2009 for the course BIO Genetics taught by Professor Saxena during the Fall '08 term at University of Texas.
- Fall '08