lecture_3__05 - Three models of DNA replication: Fig. 16.8...

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Fig. 16.8 Three models of DNA replication: Correct model: Each strand of the original template becomes part of a new double-helix
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Meselson-Stahl experiment, 1958 Meselson and Stahl made use of the fact that DNA could be labeled with different isotopes of nitrogen, and DNA containing varying ratios of the nitrogen isotopes could be separated using centrifugation. green = N (heavy) 0 1 2 rounds of replication: = > > see Fig. 16.9 www.blc.arizona.edu/marty/411/Modules/msexp.html
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1st round of replication: only one product 2nd round of replication: two products, new one is lighter than the first heavy light 4th round of replication: primarily the light product P.N.A.S ., 44 , (1958), 671-682) www.biology.arizona.edu/molecular_bio/problem_sets/nucleic_acids/graphics/ (why mix these?)
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Fig. 16.10 linear chromosomes initiate replication at many sites (origins of replication) replication proceeds in both directions from each origin; generating 2 “replication forks”
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Fig. 16.13
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lecture_3__05 - Three models of DNA replication: Fig. 16.8...

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