lecture_18__05 - Bacterial genetics Transduction:movement...

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Bacterial genetics Transduction :movement of genetic information from one bacteria to another via bacteriophages. 2 types: Fig. 18.13 "generalized": transfer of bacterial genes randomly packaged into phage head during lytic cycle “co-transduction” refers to the transfer of multiple cellular genes via generalized transduction. The closer the cellular genes are to each other in the bacterial chromosome, the greater the likelihood of co-transduction
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Fig. 18.13 "specialized": transfer of bacterial genes located adjacent to bacteriophage genome during the lysogenic cycle QuickTime ? and a Photo - JPEG decompressor are needed to see this picture.
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Imagine that a bacteriophage is created with a mixture of materials from 2 different phages (T2 and T4) such that the outer protein coat of the new phage is derived from T2, but the DNA is derived from T4. If this engineered T2/T4 phage is used to infect E. coli and then produces new phage, this will have: a. the protein of T2 and the DNA of T4 b. the protein of T4 and the DNA of T2 c. a mixture of the DNA and proteins of both phages d. the protein and DNA of T2 e. the protein and DNA of T4 Genes percent co transduction A-C 4 A-D 18 B-A 6 B-C 12 B-D 8 C-D 6 You are working on mapping a bacterial chromosome by using a general transducing virus. The results are below. Draw a map of the 4 genes in the chromosome.
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Transformation : uptake of foreign DNA from the environment Some bacterial strains have the ability to actively bring DNA in from the environment using specialized membrane on their extracellular surface. In some cases, these proteins are induced under conditions of limited nutrients. Other strains (for instance E. coli) can be induced to take up DNA by treatment with calcium or other
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lecture_18__05 - Bacterial genetics Transduction:movement...

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