lecture_21__05 - Restriction Fragment Length Polymorphisms...

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Unformatted text preview: Restriction Fragment Length Polymorphisms (RFLP): Homologous chromosomes often differ slightly in DNA sequence (usually more changes outside of open reading frames). Some of these differences will result in different patterns of cutting by restriction endonucleases. Fig. 20.9 Electrophoresis : separation of molecules (nucleic acids or proteins) by size allele 1 allele 2 y y x w z The non-coding parts of the human genome exhibit a high level of polymorphisms. Therefore, any individual is unlikely to have the same polymorphisms as another individual. For this reason, RFLP analysis can be used to unambiguously identify a particular person. For legal purposes, several different loci are used to unambiguously identify an individual. The loci are chosen specifically for high levels of variation within the human population. Example: RFLP analysis of blood from a crime scene shows that the blood belongs to someone with the genotype D1/D3, E2/E5, F5/F5, G1/G3 (where D, E, F, and G are different loci and the numbers represent different alleles). The frequencies of these genotypes in the Caucasian population are D1/D3= 0.01, E2/E5=0.02, F5/F5=0.001, G1/G3=0.00001. If all the loci are unlinked, what proportion of the caucasian population shares this genotype with this person? A) 0.311 B) 0.0001 C) 0.00005 D) 0.000001 E) 2 x 10-12 the most common uses of RFLP analysis are identification of criminal suspects and paternity cases. An RFLP analysis is sometimes called a DNA fingerprint . These DNA fingerprints represent 4 different individuals. Which of the following statements is consistent with the results? A) B is the child of A and C B) D is the child of B and C C) A is the child of B and C D) C is the child of A and B E) A is the child of C and D DNA sequencing: To determine the sequence of a particular DNA molecule, the molecule is used as a template...
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lecture_21__05 - Restriction Fragment Length Polymorphisms...

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