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Unformatted text preview: Paull BIO 212 Problem Set #10 (12 pts) Due on April 21/22 RFLP/sequencing/population genetics 1. (2 pts) Claude is doing a standard DNA sequencing reaction but makes a mistake and uses regular deoxynucleotides instead of dideoxy nucleotides. What does Claudes sequencing gel look like when he develops it? 2. RFLP analysis can be used to correlate genotypes with phenotypes in humans. Shown below is a pedigree with corresponding RFLP data directly below. Part a (1 pt): which of the bands in the RFLP analysis are correlated with the disease phenotype? c and g Part b (2 pts): which of the individuals in the pedigree are carriers of the disease allele but are not showing the disease phenotype? 1.1 and 2.2 no bands in the gel; all the radioactivity at the top of the gel because the polymerization reactions continued without stopping to the end of the template. These long products will all be up at the top of the gel....
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