BIO211Ch7.Cell_StructureandFunctionBB

BIO211Ch7.Cell_StructureandFunctionBB - Cell Structure and...

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    Cell Structure and Function Outline Methodology Overview Nucleus Cytoplasmic contents Cell surface
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    How to study cells? Structure 1. Microscopy Light Microscope: Brightfield, Darkfield, Phase Contrast, Differential Interference and Confocal. Electron Microscope: TEM, SEM 2. Cell fractionation and Gel electrophoresis
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    Methodology (contd.) Function      1. Cell fractionation and   Gel electrophoresis       2. Biochemical assays
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    Units of Measurement Microscopy magnification resolution Light microscope 1000 X, 0.2  μ m Electron Microscope   100,000 X, 1 - 2 nm   X-ray Crystallography  for 0.1  0.2 nm
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    Microscopes
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    Light Microscope Images Brightfield       unstained Brightfield       stained Fluorescence Phase contrast Differential interferrence Confocal
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    Electron Micrographs Transmission EM Scanning EM
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    Cell Fractionation Selection of tissue Selection of procedures Buffers, Centrifugation, Assays.
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    Types of Centrifuges Clinical centrifuge (1- 5 k RPM) Microfuge (14 k RPM) Floor model or desktop centrifuge (1- 20 k RPM) Ultracentrifuge (1- 80 k RPM) Rotors Fixed angle and swing bucket
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    Gel Electrophoresis Agarose gels: DNA, RNA Polyacrylamide gels: Proteins
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    Overview of cells Prokaryotic  Cells Eukaryotic  Cells Plant Cells Animal  Cells
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    Animal Cell
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    Plant Cell
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    Why are most of the cells so microscopic  and compartmentalized?
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BIO211Ch7.Cell_StructureandFunctionBB - Cell Structure and...

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