Lecture 4 - BIS101-001 Lecture 4. 1/14/09 Complementation...

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Unformatted text preview: BIS101-001 Lecture 4. 1/14/09 Complementation Gene Interactions Linkage Podcasts for BIS101 How to subscribe: http://podcast.ucdavis.edu Download: http://podcast.ucdavis.edu/app/view.php?pid=398 LECTURE 4: OUTLINE Complementation Gene interactions Complementary gene action Recessive epistasis Recessive suppression Duplicate genes Linkage Morgan s demonstration of linkage Two point cross Crossing over Mapping by recombination frequency If many genes are involved in a process, many mutations will give the same phenotype How can we tell if 2 mutations are in the same gene, or in different genes? starting metabolite intermediate fnal product enzyme 1 enzyme 2 Complementation: Production of a wild-type phenotype when haploid genomes bearing two different recessive mutations are brought together in a diploid cell. e.g. Parents aa BB x AA bb white Fowers white Fowers 1 A a B b purple Fowers because two different genes (A and B) are both needed to make the purple Foral pigment Complementation with Drosophila eye colour mutants- Buff-- Apricot--- Coral---- Cherry + + + +- Vermillion + + + + +- Ruby + + + + + +- Garnet---- + + +- White Buff Apricot Coral Cherry Ver- million Ruby Garnet White CROSS Complementation with Drosophila eye colour mutants- Buff-- Apricot--- Coral---- Cherry + + + +- Vermillion + + + + +- Ruby + + + + + +- Garnet---- + + +- White Buff Apricot Coral Cherry Ver- million Ruby Garnet White CROSS Complementation with Drosophila eye colour mutants- Buff-- Apricot--- Coral---- Cherry + + + +- Vermillion + + + + +- Ruby + + + + + +- Garnet---- + + +- White Buff Apricot Coral Cherry Ver- million Ruby Garnet White CROSS Complementation with Drosophila eye colour mutants- Buff-- Apricot--- Coral---- Cherry + + + +- Vermillion + + + + +- Ruby + + + + + +- Garnet---- + + +- White Buff Apricot Coral Cherry Ver- million Ruby Garnet White CROSS 1 1 1 1 1 Complementation with Drosophila eye colour mutants---------- + + + + + + + + + + + +---- Buff Apricot Coral Cherry Buff Apricot Coral Cherry- Vermillion +- Ruby + +- Garnet + + +- White Ver- million Ruby Garnet White CROSS Complementation with...
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Lecture 4 - BIS101-001 Lecture 4. 1/14/09 Complementation...

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