Lecture 7 - BIS101-001 Lecture 7. 2/2/09 Bacterial genetics...

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BIS101-001 Lecture 7. 2/2/09 Bacterial genetics continued Using genetics to study gene structure and function
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- Review quiz: mean 32.4, median 33 - Pick up midterm 1 after Wednesday in boxes outside MCB office.
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LECTURE 7: OUTLINE Review: bacterial gene mapping by conjugation Gene transfer in bacteria: 2) Transformation 3) Transduction with bacteriophages Using genetics to study gene structure and function One gene encodes one polypeptide Gene mutations cause protein changes Fine mapping to analyze gene structure
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A cross is made in E. coli between an Hfr strain that is leu+ his + gly+ Str s and an F- strain that is leu- his- gly- Str R . Interrupted mating studies show that his+ enters the recipient last. In an interrupted mating between the same two strains, what medium would you select the conjugates on before screening for recombinants? A. Medium containing his and lacking streptomycin B. Medium containing his and containing streptomycin C. Medium lacking his and containing streptomycin D. Medium lacking his and lacking streptomycin
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E. coli between an Hfr strain that is leu+ his+ gly+ and an F- strain that is leu- his- gly- . Interrupted-mating studies show that his+ enters the recipient last. In an interrupted mating between the same two strains, his+ recombinants are selected and tested by replica plating for the presence of the leu+ and gly+ alleles. The following numbers of individuals are found for each genotype: (1) gly+ leu+ his+ = 259 (2) gly- leu+ his+ = 1 (3) gly+ leu- his+ = 11 (4) gly- leu- his+ = 29 ---- 300 What are the gene order and map distances between these three markers? A.
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Lecture 7 - BIS101-001 Lecture 7. 2/2/09 Bacterial genetics...

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