Lecture 8 - BIS101-001 Lecture 8 2/4/09 DNA Structure...

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Unformatted text preview: BIS101-001 Lecture 8 2/4/09 DNA Structure & Replication # of students 0- 10 10- 20 20- 30 30- 40 40- 50 50- 60 60- 70 70- 80 80- 90 90- 100 Midterm 1 mean = 73.5 median = 77 s.d. = 15.6 50 40 30 20 10 You have isolated 3 phage T4 rIIA mutants (a-c) that you wish to map. You obtain 3 different rIIA deletion mutants from Dr. Benzer (as shown below) and perform intragenic recombination studies by co-infecting each mutant with each deletion in E. coli B and then determining if you isolate wild type recombinant phage by infecting in E. coli K. You obtain the following results (- = no wild type recombinant phage generated; + = wild-type recombinant phage are generated): 1 2 3 a- - + b- + - c- - - Deletion 1 ________( )_____________ Deletion 2 _( )__________________ Deletion 3 ______________( )_____ Mutant a maps: A. _____________a __________ B. ___a ____________________ C. ________a _______________ You have isolated 3 phage T4 rIIA mutants (a-c) that you wish to map. You obtain 3 different rIIA deletion mutants from Dr. Benzer (as shown below) and perform intragenic recombination studies by co-infecting each mutant with each deletion in E. coli B and then determining if you isolate wild type recombinant phage by infecting in E. coli K. You obtain the following results (- = no wild type recombinant phage generated; + = wild-type recombinant phage are generated): 1 2 3 a- - + b- + - c- - - Deletion 1 ________( )_____________ Deletion 2 _( )__________________ Deletion 3 ______________( )_____ Mutant b maps: A. ____________ b __________ B. ___b ____________________ C. ________b _______________ You have isolated 3 phage T4 rIIA mutants (a-c) that you wish to map. You obtain 3 different rIIA deletion mutants from Dr. Benzer (as shown below) and perform intragenic recombination studies by co-infecting each mutant with each deletion in E. coli B and then determining if you isolate wild type recombinant phage by infecting in E. coli K. You obtain the following results (- = no wild type recombinant phage generated; + = wild-type recombinant phage are generated): 1 2 3 a- - + b- + - c- - - Deletion 1 ________( )_____________ Deletion 2 _( )__________________ Deletion 3 ______________( )_____ Mutant c maps: A. ____________c ___________ B. _______( c )_______ C. ________c _______c _______ D. Both B and C are correctD....
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Lecture 8 - BIS101-001 Lecture 8 2/4/09 DNA Structure...

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