Polyacrylamide Gel Electrophoresis (PAGE)
Under Denaturing Conditions
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Polyacrylamide Gel Electrophoresis (PAGE)
Under Denaturing Conditions
University of Texas at Austin Department of Chemistry and Biochemistry
Introduction
Gel electrophoresis allows charged molecules to migrate through a solution once a
current is placed going through the gel. The way the molecules migrate down the gel, due to
charge density, can be used to separate and determine molecules. The gels used for
electrophoresis can vary by pore size, allowing the user to construct the experiment to their
standards. The gels can also vary by type; starch, agarose, and polyacrylamide are common types
of gels. We used a polyacrylamide gel because they are great for proteins and can achieve very
high resolution. The addition of a sample loading buffer to each of the samples allowing the
denaturation of the proteins to occur; it prevents disulfide bond formation and prevents multi-
subunit complexes.
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- Spring '09
- GeneMcDonald
- Chemistry, molecular biology, Electrophoresis, pH, SDS-PAGE, Molecular Marker Molecular Marker Molecular Marker Rf
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