Lab 6 - Isolation and PCR of plasmid DNA and using Agarose...

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Isolation and PCR of plasmid DNA and using Agarose Gel Analysis to Analysis Plasmid and Human DNA 1
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Isolation and PCR of plasmid DNA and using Agarose Gel Analysis to Analysis Plasmid and Human DNA University of Texas at Austin Department of Chemistry and Biochemistry Introduction Scientists want to analyze not just our own DNA but also foreign DNA as well. The use of cloning allows foreign DNA to be replicated and expressed inside the cells of another species. The use of plasmids, small circular DNA that are mostly found in bacteria, are using as a cloning vector. They are ideal because they contain a replication origin, restriction site for insertion, and a selection gene. To obtain these plasmids, you must harvest host cells (E.coli used), lyse and remove unwanted proteins, and precipitate the plasmid DNA. PCR, polymerase chain reaction, is an in vitro method of DNA and RNA amplification. Only the sequence region between the primer sites of the DNA/RNA is amplified. It is used in forensic analysis, paternity testing, and other important uses that
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This note was uploaded on 09/07/2009 for the course CH N/a taught by Professor Genemcdonald during the Spring '09 term at University of Texas.

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Lab 6 - Isolation and PCR of plasmid DNA and using Agarose...

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