Lab 7 - Transformation and Plating of E.Coli, Examination...

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Transformation and Plating of E.Coli, Examination of Transformed Cell Cultures, and Purification of a Cloned Protein 1
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Transformation and Plating of E.Coli, Examination of Transformed Cell Cultures, and Purification of a Cloned Protein University of Texas at Austin Department of Chemistry and Biochemistry Introduction E.Coli are types of cells that are used for many experiments. They are a type of bacteria that are capable of transferring DNA via bacterial conjugation. The E.Coli cells are used in our experiment to observe the transformation of cell cultures and help to purify the His-tagged protein via IMAC, immobilized metal affinity chromatography. The transformation process involves the uptake of a vector with the gene of interest. Upon transformation, the E.Coli cells will replicate the DNA and this new DNA will now be spread into all of the resulting colonies. IMAC, is a type of column chromatography where amino acids covalently bond to metals, histidine in our case.
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Lab 7 - Transformation and Plating of E.Coli, Examination...

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