seq_ex_sp09_key - 98, 53 6. Compare the nucleotide sequence...

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CH369L/387K Name________ KEY __________ Spring 2009 Lab section________________ Genomics exercise 5’- ttaaggaaga acaatgattc ttaaaattct gaacgaaata gcatctattg gttcaactaa acagaagcaa gcaattcttg aaaagaataa agataatgaa ttgcttaaac gagtatatcg tctgacttat tctcgtgggt tacagtatta tatcaagaaa tggcctaaac ctggtattgc tacccagagt tttggaatgt tgactcttac cgatatgctt gacttcattg aattcacatt agctactcgg aaattgactg gaaatgcagc aattgaggaa ttaactggat atatcaccga tggtaaataa -3’ Using Gene Runner, analyze the DNA sequence above and answer the following questions: 1) How many restriction sites are there in this sequence? 55 2) At what position (base number) in the sequence is(are) the HaeIII site(s) located? 163 3) At what position (base number) in the sequence is(are) the MseI site(s) located? 1, 21, 105, 281 4) How many open reading frames (ORFs) >75 nucleotides are there in the sequence? 2 5) For each ORF, (a) in which reading frame is the ORF located? +2, -2 (b) how many amino acids does the ORF code for?
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Unformatted text preview: 98, 53 6. Compare the nucleotide sequence above to the Blast nucleotide sequence database (blastn using nr database). Of what gene is the sequence most likely a part? Phage T4 ligase Genomics exercise ggtlaiqaqg dltlaqkkiv rktwhqlmrn ktsfvtdvfi rifaydpsaq nkfpqmagms asqlrssrqm qahairvssi mseyveelds dilpellatl arthdlnkvg adhynlfakv lmealqaelg sdfnektrda wakafsvvqa vllvkhgn Using Gene Runner, analyze the protein sequence above and answer the following questions: 7. How many potential cleavage sites for chymotrypsin are there in this sequence? 12 8. What is the calculated isoelectric point (pI) of this protein? 8.99 Compare the protein sequence above to the Blast protein sequence database (blastp using nr database). 9. What is the most likely identity of this protein? Globin C, coelomic 10. In what journal was this sequence originally published? Journal of Protein Chemistry (J. Prot. Chem.) 2...
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seq_ex_sp09_key - 98, 53 6. Compare the nucleotide sequence...

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