wgap_ex_sp09_key

wgap_ex_sp09_key - filtration step? 1980X 4. According to...

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CH369L/387K Name_______ KEY ___________ Spring 2009 Lab section________________ WGAP literature exercise Read the WGAP primary reference paper (Waymack and Van Etten 1991, Arch. Bioch. Biophys . 288 , 621) and answer the following questions. 1. What was the concentration and pH of the acetate buffer used by Waymack and Van Etten for the initial extraction of wheat germ? 0.3 M, pH 4.0 2. What concentration of NaCl resulted in elution of the most WGAP activity from the SP- Sephadex ion exchange column? 0.23 M 3. What purification factor was achieved by Waymack and Van Etten after the G-75 gel
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Unformatted text preview: filtration step? 1980X 4. According to this paper, how many phenylalanine residues are there in WGAP? 15 5. According to this paper, what is the pH range of maximal stability of WGAP? 4-7 6. According to this paper, what is the molecular weight of WGAP, based on a) gel filtration chromatography 60,000 and 58,000 Da b) SDS-PAGE electrophoresis 56,000 ± 3000 Da c) amino acid analysis 54,950 Da 7. What value did the authors obtain for K m of WGAP at pH 5.0? 0.09 ± 0.01 mM 8. What value did the authors obtain for V max of WGAP at pH 5.0? 823 ± 30 mmol mg-1 min-1...
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This note was uploaded on 09/07/2009 for the course CH N/a taught by Professor Genemcdonald during the Spring '09 term at University of Texas.

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wgap_ex_sp09_key - filtration step? 1980X 4. According to...

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