Exam_1_s05 - Spring '05 Hackert CH370 / CH387D Exam I Name...

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1 Spring '05 CH370 / CH387D Name ________________________ Hackert Exam I 1 . Identify each amino acid by its three and one letter codes (e.g. Ala / A , etc. ) and each N base, nucleoside or nucleotide by its full name in the blanks ABOVE the structures (26 pts). (26) H 3 N + CH COO - H H 3 N + CH COO - CH 2 HS H 3 N + CH COO - CH 2 H 2 C COO - H 3 N + CH COO - CH 2 H 3 N + CH COO - CH 2 - OOC H 3 N + CH COO - CH CH 2 H 3 C CH 3 H 3 N + CH COO - CH 2 HO H 3 N + CH COO - CH 3 H 3 N + CH COO - CH 2 HN + NH H 3 N + CH COO - CH OH H 3 C H 3 N + CH COO - CH 2 H 3 C CH 3 H 3 N + CH COO - CH 2 H 2 C S H 3 C H 3 N + CH COO - CH H 3 CC H 3 H 2 N + CH COO - CH 2 CH 2 H 2 C H 3 N + CH COO - CH 2 H 2 C CH 2 H 2 C 3 + H 3 N + CH COO - CH 2 H 3 N + CH COO - CH 2 H 2 C C H 2 N O H 3 N + CH COO - CH 2 H 2 C CH 2 CH H 2 N 2 + H 3 N + CH COO - CH 2 C H 2 N O H 3 N + CH COO - CH 2 N O O H 3 C A) B) C) D) E) F) G) H) I) J) K) L) M) N) O) P) Q) R) S) T) U) W) X) Y) Z) V) O = O 3 PO N N NH 2 O H ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ ____________ N NH O O O N N N N 2 H NH N N N O 2 H N N N N 2 O = O 3 PO
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2 2. a) Which one amino acid is primarily responsible for the adsorption by proteins at 280 nm? (2) _____________________________________ b) Identify the 3-letter code of one amino acid for the approx. pKa’s listed for their side chains. (5) 4: ____ 6: ____ 8: ____ 10: ____ 12: ____ 3. You are trying to purify a mixture of the following two polypeptides: RAMACHANDRAN and TEAMLEADER a) Estimate the charge of each peptide at pH 14.
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This note was uploaded on 09/07/2009 for the course CH 53575 taught by Professor Hackert during the Spring '09 term at University of Texas at Austin.

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Exam_1_s05 - Spring '05 Hackert CH370 / CH387D Exam I Name...

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