Lecture 7

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hapter 5 Calibration Methods Chapter 5 Calibration Methods A major goal of quantitative analysis is to determine the oncentration of nalyte 1. Calibration Curves concentration of analyte. Calibration Curve: Standard solution: Blank solution: inear range of a calibration curve: Linear range of a calibration curve: Dynamic range of a calibration curve:

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= 0 01630 ( 00022)X +0 0047 ( 0026) Y = 0.01630 (±0.00022)X +0.0047 (±0.0026) Example: An unknown sample gave an absorbance of 0.406, and a blank had an absorbance of 0.104. How many micrograms of protein are in the unknown?

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Unformatted text preview: 2. Standard addition Standard addition: Matrix effect: Example: Serum containing Na+ gave signal 0f 4.27 mV in an atomic emission analysis. Then 5.00 mL of 2.08 M NaCl were added to 95.0 mL of serum. This spiked serum gave a signal of 7.89 mV. Find the original concentration of Na+ in the serum. tandard addition: Standard addition: 3. Internal standards Internal standards: Response factor: Example: Ax = 553 and As=582. Suppose response factor, F =0.7900, and [S] = 0.0584 M. Please calculate [X]. k Homework: 5 ‐ 22, 5 ‐ 23, 5 ‐ 28, and 5 ‐ 29....
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