Lecture 21 - Chapter Ch t 19 and 20 d Application of...

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Chapter 19 and 20 Application of Spectrophtometry and Spectrophotometers
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Application of Fluorescence Spectroscopy Example: In a RNA assay, the fluorescence intensity of the chip is linearly dependent upon the concentration of RNA in a range from 1.00 μ M to 1.00 nM. A standard solution with a RNA concentration of 1.00 μ M leads to a fluorescence response of 1.00 x 10 6 counts, and a solution of RNA concentration of 2.00 nM gives a response of 1.00 x 10 4 counts. An unknown RNA sample give a fluorescence response of 6.00 x 10 5 counts. Please calculate the RNA concentration in this unknown sample. If this RNA concentration is identical to the RNA concentration in a cell (6 μ M in diameter), calculate how many such RNA in such a cell.
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Time-Resolved Fluorescence Spectroscopy
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Luminescence Resonance Energy Transfer (LRET) bio targets LRET Donor: Mn doped QDs; cceptor: dye molecules (e g cy5) labeled on the bio rgets E = n R 0 6 Acceptor: dye molecules (e.g., cy5) labeled on the bio targets E: rate of LRET, n: number of acceptors interacting with a single donor, R 0
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Lecture 21 - Chapter Ch t 19 and 20 d Application of...

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