review natalia

review natalia - Lab Exam Review Tuesday, 11/18/2008 BISC...

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Lab Exam Review Tuesday, 11/18/2008 BISC 320-L TA: Natalia
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DNA Profiling - PCR Alu sequence SINES RNA-mediated transposition Dimorphic DNA collection method QIAamp Spin Column Polymerase Chain Reaction Interpretation of PCR and gel results
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Polymerase Chain Reaction Watson, JD et al,. Molecular Biology of the Gene , Pearson Education, Inc. San Franscisco, 6th Ed. Strategene Robocycler Gradient 96 Reaction Mix: 20 μ l of DNA template (from cheek cell) 20 μ l of PCR Master Mix ( Taq polymerase, primers, dNTPs – A,T,G,C) PCR cycles: 40 cycles: 1 min at 94°C (denaturation) 1 min at 64°C (annealing) 2 min at 72°C (extension)
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Expected Results 941bp 641bp Alu -300bp intron intron Primer 1 Primer 2 +/+ : 941bp -/- : 641bp ( )
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Transformation of E.coli (I) Bacteria vs. plasmids Qualities required for plasmid to be used in molecular cloning Competency and its induction Transformations 2 methods Steps and function of each treatments
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This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

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review natalia - Lab Exam Review Tuesday, 11/18/2008 BISC...

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