08 320 lab exam sample questions

08 320 lab exam sample questions - Fall 2008 BISC 320...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Fall 2008 BISC 320 Laboratory Final Exam Sample questions Question 1 : You were given 60 μ l of 800 base pair of PCR products. In order to quantify the concentration, you diluted 4 μ 1of PCR products with 198 μ 1of H 2 O. Then you took spectrophotometric measurements using the diluted sample and obtained the results below: Abs @ 260 nm: 0.66 Abs @ 280 nm: 0.10 a) What is the final concentration of your PCR products? Show your calculation and include unit (s). b) Assess the purity of your PCR product. c) If you were to first heat the PCR product at 95°C for 5 minutes and then take the spectrophotometric measurements, would the 260 nm absorbance be lower or higher than the one stated in the question? Briefly explain your answer. Question 2 a) Briefly state 2 main characteristics of Alu elements. One of them should be related to the propagation of Alu elements. b) You want to use PCR to amplify a partial region of a double-stranded DNA template. If you
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

Ask a homework question - tutors are online