Lab 2 DNA Profiling PCR

Lab 2 DNA Profiling PCR - Lab #2 - DNA Profiling Objective:...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Lab #2 - DNA Profiling Objective: to determine genotype for Alu insertion and perform Hardy-Weinberg analysis of class result Collect cheek cells 1. Each student needs to collect one sample (no food or drink 30 min prior to sampling). 2. Scrape each cheek 15 times with a Omni Swab. 3. Air dry for a minimum of 30 minutes under the hood (ends up and label) The Alu sequence is an example of a widespread short interspersed nuclear elements (SINEs) found in the human genome. Origin of Multiple Copies of Alu Sequences by Transposition Alu sequence Size: 300 bp Account for ~ 10% of the human genome, with about 1/2 million copies Alu repeat is variable but contain a small sequence that is unique to humans. No known cellular function; but good genetic marker for relatedness among individuals. The Alu sequence within PV92 locus on chromosome 16 is dimorphic . Homozygous (+/+): sequence in both chromosomes Heterozygous (+/-): sequence in only one chromosome Homozygous (-/-): sequence absent Procedure...
View Full Document

Page1 / 13

Lab 2 DNA Profiling PCR - Lab #2 - DNA Profiling Objective:...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online