Lab 3 DNA electrophoresis

Lab 3 DNA electrophoresis - Quiz Q1 Why do we use controls...

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Quiz Q1 : Why do we use controls in our experiments? Which controls will you use today? Q2 : Do you think that the allele/genotype frequencies we will observe today will exactly match the results of Hardy-Weinberg analysis? Why? Why not? (3pts)
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Please bring your STUDENT ID and a PEN if you wish to submit a re-grade. •Room assignments - You have been assigned to a room by alphabetical order of your last name. Please go to the room you are assigned. Otherwise, there is a possibility of penalty if you go to the wrong room. •If possible, we would like you to take the exams sitting in every other seat. Fall 320 Exam 1 room assignment Group Presentation Project (October 28 ) - Groups of 2 people - Scientific paper in molecular biology - Submit choice of paper October 7. Room # Students THH 101 A - R WPH B27 S - Z Room # Students THH 101 A - L SAL 101 M - Z 12pm lecture 11am lecture
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DNA Electrophoresis Objective: to run electrophoresis of PCR amplified Alu insert to determine genotype for Alu insertion to perform Hardy-Weinberg analysis of class result
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Principle of Gel Electrophoresis
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Electrophoresis Overview Load samples in conservative wells
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Agarose Gel Matrix
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Visualizing the gel with ethidium bromide Ethidium bromide is very sensitive. toxic; maybe a mutagen. When exposed to ultraviolet light, it will fluoresce with an orange color.
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Fast Blast DNA Stain product of Bio Rad Laboratories positively charged dye molecule safe and nontoxic alternative to ethidium bromide stains DNA deep blue Play DNA electrophoresis movie
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Documenting results using the Red TM http://www.alphainnotech.com/redimager/
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Expected Results 941bp 641bp Alu -300bp intron intron Primer 1 Primer 2 +/+ : 941bp -/- : 641bp ( )
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Lab 3 DNA electrophoresis - Quiz Q1 Why do we use controls...

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