Lab 4 transformation week 1

Lab 4 transformation week 1 - Lab#4 Transformation Week#1...

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Lab #4 Transformation Week #1 Today’s lab will continue for 4 weeks. Week 1 (Lab #4, part I, pre-lab and quiz only) Transform plasmids into E. coli cells Week 2 (Lab #4, part II, post-lab only) Calculate transformation efficiency Inoculate transformed colonies for plasmid propagation Week 3 (Lab #5) Isolate transformed plasmid Restriction enzyme digest isolated plasmid Week 4 (Lab #6) Confirm plasmid identity by restriction mapping
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Today’s Objectives: To understand how plasmid vector is used in molecular biology. To transform DH5 α E. coli cells with 2 types of plasmid DNA pGEM pGEM- luc
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Bacteria vs. Plasmids: E. coli E. coli Chromosome Chromosome E. coli bacteria Circular chromosome about 4.6 million base pairs. Plasmids Much smaller, 1 to 200 thousand base pairs. Usually carry useful genes such as ones for antibiotic resistance. Plasmids
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This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

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Lab 4 transformation week 1 - Lab#4 Transformation Week#1...

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