Lab 4 transformation week 2

Lab 4 transformation week 2 - Presentation Deliverables:...

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Presentation Deliverables: Week of Oct 6 Submit your choice of the scientific paper for approval. Week of Oct 27 Present the paper in lab Mac users should convert their presentations to PC format, you will be using the lab pc.
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Lab #4 Transformation week #2 Objectives: to inoculate transformed cells for plasmid propagation to calculate transformation efficiency
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Overview of Today’s Experiment LB/A/X plate for picking one blue and one white colony. And calculating transformation efficiency LB/A and LB/A/X should have similar number of colonies. Transformed Plates from Last Week LB LB/A LB/A/X blue colony white colony
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Empty Vector Ampicillin Plate Recombinant Vector Plasmids Transformed Last Week β -galactosidase blue colonies truncated β -galactosidase white colonies
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The Lac Z Reporter Gene β -galactosidase 5’ 3’ lac Z gene N C (natural substrate) Lactose glucose + galactose β -galactosidase X-gal insoluble blue compound β -galactosidase (artificial substrate) (non-diffusible, nontoxic)
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This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

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Lab 4 transformation week 2 - Presentation Deliverables:...

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