Lab 5 restriction_digest

Lab 5 restriction_digest - Mid-Semester TA evaluations TAs...

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Mid-Semester TA evaluations TAs should not handle completed evaluations. A designated student should submit the completed evaluations to ZHS 357 or 353. If no one is present, slide the forms under the door.
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Announcements: Exam Regrade Request Due today! Group Oral Presentation October 27, 2008 Mac-PC compatibility 2 persons/group or other approved arrangement November 14, 2008 Last day to drop with a W
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Lab #5 Plasmid Isolation & Restriction Digest Objectives: to isolate pGEM and pGEM- luc from bacterial cells to perform restriction digest Note: page 42 of your lab manual indicated that you will calculate the transformation efficiency again. You will not be doing that.
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Last Week: Cultured overnight Centrifuged to pellet cells. Stored at 4 o C. single colony single colony
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DNA Isolation by Alkaline Lysis Goal to free the plasmid DNA from the cell, leaving behind the E. coli chromosomal DNA, cell wall debris, proteins and other cell components. Method: differential precipitation
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This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

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Lab 5 restriction_digest - Mid-Semester TA evaluations TAs...

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