2004 MT3

2004 MT3 - Last Name(print) m I First Name(print) - c—fi...

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Unformatted text preview: Last Name(print) m I First Name(print) - c—fi RLab Section (time and day) TAs g“ ( 7 BISC 320 Midterm Exam 3 — 11/10/04 Total Score (out of 100 pts) 23 Please note: if you use pencil or whiteout, you will have NO chance for a re-grade. / 1. (10 pts) How is sigma 70 protein utilized in initiation of transcription in prokaryotes? glgma '10 wmcm m9 m W/ afiachmm’f 0? RNRPWMWM90 t4) mRNA. same! '10 Wan and: m 1110 I729 —» -10 b? Mmny gap/Wm. W W RNA Mmmw WW 90 mo crew 70 pvrvom. -lb glgmqw LbMA/pg mm pol/3me fwd WW {mm/5W ulqMC/U 2. (10 pts) How is the carboxy terminal domain (CTD) of the large subunit of eucaryotic RNA polymerase II utilized during transcription? cw cmrams we www.733qlmaés of 7 MIA/Mu nails. If naming Walsh 06 W-WW WVWM MWHWD 06424 ls VMbSpLj/dmmkd, 11w . LTD com/MS p m MW WV gel/I01 6W mRNA.\A.W mo mgfln WWII/ID mm xswnwv, ' 3 9mm \8 llqnyhy Mam/m 1w W leM 1141/ UR) W m lollle WW Mum WA fw mamfl W, in MP. We up mL '13 alon Mpmvsplmmw 1;» WM VWW Wm max/Wowng WMWA small ’ Name (print) ‘I First Name (print) _-__ fl. (lOpts) Briefly, how (_by what mechanism) do lariat structures form in splicing ‘pre—mRNA? ‘_ / Law“ WAC/Mm form by making m S‘e 2\ WWW,- me +W/ S‘ spw w M m mm and rm 2‘ 0H of w Wit/w. / , ‘ 7% 9m. UZSM RNP was WW ‘3‘ 5W” “to . ' * “BMW WW 5‘ fiumw “mm by Wflomwoh pow WWW / vmu’m, MagRNP WM owes the 3‘ WW symw ng 4. (10 pts) Of what value is an SR protein that binds to an ESE sequence within an exon in a pre—mRNA strand? 92 Mum 'bmdmg «N am 696 mamas wwpwswm of W, (My; . ' EQE smms (cw Exam $9M om What/Ida". sp wan/ls WM! 9W; ESE (3 MWulggvmbmg and wmvtm Ww gpuwowww WWW WW ‘3thme scqvwnw, (WWI/3M a Mr K WM. »- 9R mums aw \WWUYWMW WWI/L6 If M “(m/MW ‘5 (WWW 0v wt 75?? Name(print) ‘ First Name(print) - 3'5. (10 pts) In post—transcription editing of mRNA, how can 5 deamination change a sense codon into a translation stop codon? m Acaw'xmmmm, cymvw mwmaw minnows om mum gun/1;? ) Aw 04mm,me mung w WWW» 170 a WWW Wow . \V\ W MPNA Wand/W (MC/V1 HOW wads“ [1AA] mth am: an 0\ 3M) Mm for mm mm . ‘ime m4“ 3‘ Q0AA '3" . 9 wine Ammmcm \ O r \L C—vu S “ T AA ‘ 3' 6. (10 pts) Briefly, how is transcription termination, with position specificity, accomplished in procaryotes and eucaryotes? (Do not bother to mention any protein factors involved.) Mkawkccwexmwxowm Adm/cm; mowmmt M We \mcwpmcwn’r. . In m derW; W» ANA semi/aw Aux/«samba! (Mum—g mva sequmwsiwg NW tramsan mm mRNA,W saw/1w W \MM pmamaw. mm Qatam‘dmg \oma A mm mm, wu£ingmM_ A mm ound WWW pm W). 'W, m w am A w WW \0 mm mm aw ‘ Wgu gamma dwwWTVAfl-T W20 Pom} 0% Me As DNA .erW M w wmdxrvfgawtwm / A WWW WMWWWGG by \as 63m Y]? gfiai Dacia. {VIM/1979’ W (Mas We @0131 V!) w WI 13 r . A -: Ag ' 4‘ mm mm Wm ull MM YARN, WY: ‘ w ‘mfi-ér DNA WWme PM as mkmhliém Wéfive 3W} ‘ Wmdmg afwvmmmmv; 9+0 WW MEN: . WW3 W3 ANA WWW IL wads smug/1w AAA AAA. “Mob? Law Aw mRNA wax/1mg out I, I if] Name(print) ! First Name(print) - 7. (10 pts) (a) What is meant by the term ORF (open reading 0 frame) as it is used in protein synthesis? (b)-. How many translational reading frames are possible on the same mRNA strand? > Wflw mRNA . V @ ORF (S m own readmg W‘wwn wumlm w mums WWI ‘fiW WWW . mum WSLWS ‘ W am scat/1‘ W a ORF/WWW up w (AW on W - b—E mwmm M appr ' CD '|—‘ O '6 Ft. (D H :3 ’U H O '5 ‘V “- H \ .L< ‘ O (.r (D U) .23" O E} D; O ('D U) m. '1 DJ :3 U) H 9.) (T H. O :3 '_l. :3 H. (.1. [—1. $1) (.1. (D -\J ’ / I WWCé/fimm Wlbsr W ‘ \g \‘osrRNA - W \an am mm M 9m I! g g‘eqmw Amman/mm 80g gum/MM I": lemma/ch13) Web/A. ‘ mm IS a Pcm,Ag\u,am sst. A w Ra \Wt‘mm WA \‘g mm mm P am pm (Mr sum/m mfivs+ Lawn/o Ma WMM co-WU Is N I ( WWW, Jr l PWMS‘ MVOWEW \F mm mm m A globe WW“ l A Wu two A 9er bf W 30; SWWH ' WW? GTPhfit/xas U «W 30: gummy {Ffljm l m WNA. HWL WW'W v. came/ho w swwgmw “5 r WWAM\\V\W@.WVWEQKPW «34 SD: wbmvm’ fwmw‘ OHM- “ ' ' - MAC/4: C7 WC?! ' HEW 30$ WWWT scav'VLS’am gmykmmm . (1)?!) 1:63:63de . W WVMW’M moms. I (E m \Fl W86, / me MW GEN/i [MW 1:0 ‘51“ 99 find“ g bx .. é: E “.55 E E E D 72m; U hwy/w; ’ _ Name(print) 1 First Name(print) —' g 9. (10 pts) What is the purpose of the A, P, and E sites on ribosomes? A W “WWW cam/3M9 W ammo aa'd Vii/IOU . i7» W8 me. I ‘ < P 9M1 max-m5, WA 3mm in 1W3 9m. 9M» {W WNA Wrbh w wwx po WV cham- 6 am: 9%,?» N%& WNA Ohm um t9 mmw 'Ww PSW/ males, Mom/Lg w MWIWID' (2x341 mm a 146W MhMLWW WA av» a W mama ‘UZN/i 00m WMXW WWWV M WW oom- , " r wvmmww o Wmm 1m Pdtu‘mn, Wigwam mfww 0"“ WSW-W paw WA WW 49:3; 9% and A CW DIANA W63 u; p sue. 10. (10 pts) (a) How do stop codons manage to stop protein synthesis? (b) How do ribosomes then release from mRNA? pWWSthbfio’Mfl/W ngp ngjapmmjfllw WN- 90M ' W, Mad/M W1 as v» . . .~ WWMM we WWW WA WWIH WAS“ - me mom {Mm-Mm read WW mum. mm‘wml RF1=2was um and um RF2= mas MM and Mém WWYW:%F1“TM03 an 11mm Qnsp mam/IS CUMMMQ, um"), 3=sixrvmmks Watssomw 01° WW IZFZL and [LFZ ‘ em: Siwtha‘rcs W dismawm crf exam, - ’ pmn SW (S coma, and WWW WhWSMfl/Mom w MWA 3% mm WbayLS. W 0i? W WWW-S (1M 11%, elm/arm) urn/1mm w A gm sag-5m ’0” W a pm 0‘; W 303 and W03 swam/Ms;th WW7 PAW OMSSOWWJ mm W WSW, tum/M03qu (XIKSOWWS fwrv' w mm. I , ,‘y‘ ‘ 'l 1' ‘1: 5 ...
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This note was uploaded on 09/12/2009 for the course BISC 320L taught by Professor Baker,aparicio during the Fall '07 term at USC.

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2004 MT3 - Last Name(print) m I First Name(print) - c—fi...

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