2004 MT3 blank

2004 MT3 blank - Last Name(print m a First Name(print Lab...

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Unformatted text preview: Last Name(print) m a First Name(print) - Lab Section (time and day) TAs 9" 5 BISC 320 Midterm Exam 3 - 11/10/04 Total Score (out of 100 pts) 23 Please note: if you use pencil or whiteout, you will have NO chance for a re-grade. l. (10 pts) How is sigma 70 protein utilized in initiation of transcription in prokaryotes? 2. (10 pts) How is the carboxy terminal domain (CTD) of the large subunit of eucaryotic RNA polymerase II utilized during transcription? Name(print) _. First Name(print)-____ . (lOpts) Briefly, how (by what mechanism) do lariat structures form in splicing pre—mRNA? ; 4. (10 pts) Of what value is an SR protein that binds to an ESE sequence within an exon in a pre—mRNA strand? f vx/Namemrint) ‘ First Name(print) - A y / D. (10 pts) In post—transcription editing of mRNA, how can\\\‘J/// deamination change a sense codon into a translation stop codon? 6. (10 pts) Briefly, how is transcription termination, with position specificity, accomplished in procaryotes and eucaryotes? (Do not bother to mention any protein factors involved.) Name (print) ! First Name (print) - 7. (10 pts) (a) What is meant by the term ORF (open reading frame) as it is used in protein synthesis? (b)-. How many translational reading frames are possible on the same mRNA strand? _ _ V . 8. (10 pts) In prokaryotes, how does translation initiate? A Name(print) 1 First Name(print) -' (10 pts) What is the purpose of the A, P, and E sites on ribosomes? 10. (10 pts) (a) How do stop codons manage to stop protein I \ synthesis? (b) How do ribosomes then release from mRNA? ...
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2004 MT3 blank - Last Name(print m a First Name(print Lab...

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