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Unformatted text preview: mutant protein is just as active as the wild-type. You now repeat the Anfinsen experiment on this mutant protein: i.e, unfolded the protein with urea, reduced all the disulfide bonds, put the protein in oxidizing environment to reform disulfide bonds, and then allowed to refold by removing the Urea). What percent of the original activity would you expect to recover. (6). Note: Consider in this experiment, like in the original one, only intra-molecular disulfide bonds are formed but not inter-molecular bonds. Clearly show the logic of your calculation. You can form only 3 disulfide bonds. And the number of ways of doing this is 126.96.36.199.3.2.------------ = 105 3! 2 3 So activity recovered = 100/105 = 0.95% which is the same as the case with 8 cystines...
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- Fall '09