experiment 7 - Carlos Caceres 4/5/09 Thin Layer...

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Carlos Caceres 4/5/09 Thin Layer Chromatography Compounds were spotted and, based on their intermolecular forces with silica or the solvent, would move depending on a preference. If a compound had a stronger IMF with a solvent, it would move up the plate than a compound with weaker IMF’s. Because the compounds had to be easily seen on the plate for unique Rf values, solvents that made the compound separate throughout the plate were chosen. From the results gathered in the first part of the experiment, the best solutions to use for the unknown were 100% ethyl acetate and 92:8 ethyl acetate: acetic acid because of how far each compound moved. The other 2 solvents were discarded because they had compounds that either did not move at all or had only slight movement up the TLC plate. To identify unknown A, it was spotted along with the 4 pure analgesics and the 2 chosen solvents were used to compare. Because the unknown has a chance to act differently depending on the mobile phase, the 2 solvents were used. When each plate
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This note was uploaded on 09/14/2009 for the course BIO biology taught by Professor Meighan during the Spring '09 term at University of California, Berkeley.

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experiment 7 - Carlos Caceres 4/5/09 Thin Layer...

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