experiment 8 - Carlos Caceres 4/19/09 On plate 4, the Rf...

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Carlos Caceres 4/19/09 On plate 4, the Rf values found were compared to the values found for the 3 pure components. If a component makes a part of the adulterated sample, the plate should show it. The sample 712 showed obvious signs of having pure component 711. One of the dots on the adulterated sample had a similar Rf value to 711 and both dots showed up under UV light and Iodine while neither showed up with DNPH. It was pretty clear that one of the pure components in the sample was 711-coriander. But because 2 dots showed up on the TLC plate under the sample, there was obviously more than one component used, coming from another group. Looking at the Rf values for other groups, it was easy to find the other component. Looking at the Rf values of each individual solvent, there must have been one that had a consistent Rf value with what plate 4 had, hence the reason for using several solvents. After comparing, caraway was chosen as the other component in the sample because its Rf values were similar to those of the second dot on plate 4 for
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This note was uploaded on 09/14/2009 for the course BIO biology taught by Professor Meighan during the Spring '09 term at University of California, Berkeley.

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experiment 8 - Carlos Caceres 4/19/09 On plate 4, the Rf...

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