ex_01_sp05_key - 64mins Ki MultiQIe-Choice 3 points each...

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Unformatted text preview: 64mins Ki? MultiQIe-Choice. 3 points each For each of the following questions, circle the letter corresponding to the BES'I‘ response. 1. Which of the following INCORRECTLY pairs an organelle, or a cell part, with its function? ' A. Golgi '. Involved in packaging and processing of macromolecules for secretion or for delivery to another cellular compartment B. Lysosome : Intracellular site for digestion of macromolecules Rihosome : Site of protein synthesis 8 Nucleolus '. Site of mRNA synthesis E. Nucleus : Repository of genetic information 2. A solution with a pH of 31} would have a hydrogen ion concentration of A. (1.1 M. E. {1.91 M. 0.13131 M. D. [lfltlfll M. E. flflflflfll M. 3. What is the HCOJ'fl-hCC-‘g ratio at normal plasma pH of "L4? (pmeu : 6.1)? @ nil-3n _ in '“il c. ion n. me E. It is impossible to determine from the information given. 4. Into which functional category should leucine and saline he placed? llydro phobic, nonpolar " . Acidic G. Basic D. Polar, uncharged 5. Into which functional category should serine and glutamine he placed? A. Hydrophobic, nonpolar B. Acidic . Basic 9 Polar, uncharged Continue on: .rJ're neflpage. Page 2 s. Secondary,r structure in a protein is primarily maintained by A. Van der 1Waals interactions. hydrogen bonds. C. ionicinleraetions. D. hydrophobic interactions. 7". Which of the following is characteristic of collagen? A. It consists of a left-handed triple helix. B. It has a regular. repeating primary.r structure. C. It has high content of proline. D. It contains hydroxjrproline and hydroaylysine. @ All of the above are characteristic of collagen. E. A triacylglycerol with palmitoleie acid (16:1) at each of the three positions would he expected to have a melting point less than B. greater than C. equal to, or approximately equal to the melting point of a triacylglyeerol with palmitic acid (lozfl) at each of the three positions. 9. The Na“, K+ ATPase of mammalian plasma membranes transports A. sodium inside and potassium outside. potassium inside and sodium outside. C. hoth potassium and sodium outside. D. both potassium and sodium inside. It]. Three major tlietar}r disaecharides for humans are maltose. lactose, and sucrose. What do theyr have in common? A. All contain fructose. B. All have [31,4 glycoside honds. All are reducing sugars. All contain glucose. Continue en fin: next page. Page 3 ll. 1|Which ef the fellewing characterize the likenesses and differences ef the cell walls of Gram—pesitiye and Gram—negative bacteria? A. Each has a phesphelipid bilayer. B. Each has a peptideglyean layer. C. Each has peptides eentaining D—arniue acids. @ All ml" the feregeing are cerreet. 12. Which of the fellewing is TRUE ef nucleic acids? A. The fear majer bases of DNA are adenine. thymine, cytesine, and uracil. C19 Beth DNA and RNA have pelynucleetide chains centainin g 3'5“ phesphediester kinds. C. Ease pairing ef the watgfln'Clek type is eenfined te deuble—stranded DNA. D. In deuhle stranded DNA heth strands tun in the 5' to 3’ directieu. E. All er the feregeing are cerreet. 13. Deuble-slranded DNA with a higher G—C centent would he eXpeeted te have @ ahigher _ a lower C. the same, er appreximately the same melting temperature as a double-stranded DNA with a lewer Cir-C centent. Sheri AnswerIEssa Sectien Paint Tvalues are given in parentheses after each qttestien number. l4. {3) The internal pH of a muscle cell is 6.3, maintained primarily by a phesphate buffer system. Calculate the ratiu HEPUJJ‘HPOF at this pH. Shew yeui‘ werk. {Phespherie acid pKls. 2.15, '12, 12.4} fl “"r Candi-me an the next page. Page 4 15. {8} Sketch a curve for the titration ct" aSpartic acid with NaOH starting with aspanic acid in its fullyr protonated form. Indicate which regions are in the acidic and which are in the basic range. Put an “X” on the curve corresponding to the isoelcctric point. Base 16. {3) Calculate a standard free—energy,r change for the reaction: Glucose + ATP = Glucose Iii—phosphate + ADP using the free energies of hydrolysis in the table below. Glucose o-hos hate 2 figfif 5 — +-;3 .7 “Mai? .45.} W3 i ll". {2) Indicate by a slash where each of the following agents would he expected to cleave it polypeptide chain. (a) CNBr V L A D N{Q E R F [13) Ch ymotrypsin Ale—Val—Gly—Tyrfle u—Phe Ccminuc or: the nextpagc. Page 5 18. (4) Indicate how one could Experimentally determine the difference between facilitated difquIon and passive diffusion. F flail {If )Lfi fffl/ Cane 19. (3} Sketeh a typical eukaryotie plasma membrane, like that of a red blood cell, showing phospholipids, and a typical transmembrane protein, like glycophorin. Indicate clearly the inner and outer surface of the membrane. Label the parts. Indicate the region in which one might expect to find covalent modifications to the amine acid residuea, giving the nature of these modifications. Also give the major interaction mChflfing “'13 Protein in the membrane. 1.“: 6: W6 5} p her-PM 2i]. {3) Suppose one wishes to clone a foreign DNA sequence containing gene A. Outline the steps by which one may construct a chimeric plasmid carrying the foreign DNA. A diagram would be helpful. - ":3 AM} , 1‘} 42': Pt gwmwf ® $2.47. 7%” j iJ/ \l/ w/Scme. affaflfifi . flf‘tdx m )4 gear!) nae/€435 #M Cameras on the next page. Page 15 21. (15} Acid hydrolysis of one mine-1 of a hexapeptide gave one rornol each of the following amino aeida: Ser, Val,A1a,L}ra, Gly, Aap M r 4+ . . . Fife ‘1“ Treatment of the original peptide wlth carhoxypeptidaae yielded i—‘tla. Edman degradation of the original peptide yielded Gig. Treatment of the original hexapcptide with trypain yielded two ti‘ipeptidea. éjififit; 53 '7 One of those tripeptidea yielded Ser upon Edmau degradationgger i N j r o r’ ' When filter paper electrophoreaia at pH I51} of a mixture of the two tripeptides F E was carried out, and the paper was dried and treated with ninhydrin, one of the peptides had migrated toward the anode, and the other toward the cathode. Vida {Hf-r Give a sequence consistent with the foregoing data. Partial credit will he given for each clue that you can correctly interpret. @Llodm- S -&_f‘-fi- THIS IS THE END [IF THE EMMINATIUN. ...
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ex_01_sp05_key - 64mins Ki MultiQIe-Choice 3 points each...

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