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ex_03_sp06_key - Multiple-Choice 3 points each 60 points...

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Unformatted text preview: Multiple-Choice, 3 points each, 60 points total For each of the following questions, encircle the letter correSponding to the BEST response. 1. All but one of the following compounds can be used as a substrate for gluconeogenesis. Which one CANNOT?? A. a-ketoglutarate B. fiunarate C. citrate ® oleate . glutamate 2. Which of the following are required to make one glucose from two pyruvates? A. 2 ATP, 2 GTP 4ATP,2GTP,2NADH+2H+ . . 2 ATP, 2 GTP, 2 NAD” D. 4 ATP, 2 GTP, 4 NADI-I + 4 If E. 2ATP, 4 GTP, 4NADH+4 I-f 3. Which one of the following enzymes is NOT involved in making glycogen from glucose in the liver? A. glucokinase B. branching enzyme C. UDP-glucose pyrophosphorylase D. pho Sphoglucomutase @ phosphofi'uctokinase 4. Which one of the following enzymes is NOT involved in making glucose from two lactates? A. pyruvate carboxylase B. PEP carboxykinase C. glyceraldehyde phsophate dehydrogenase glycerol kinase E. glucose 6-phosphatase A B C D. pyridoxine ® (No cofactor is required.) 6. Consider the folllowing steps in B oxidation of a fatty acid 1 introduction of a double bond 2 oxidation of B —OH group to a B keto group 3 formation of CoA derivative . 4 thiolytic cleavage ' S. acylation of carnitine Which of the following gives the correct sequnce of events? A 1-2—3-4-5 B 5-3-2-4-1 C 4-5—3-1-2 _ D. 3- 1-24-5 ® 35-1-24 7 When oleic acxd (18:1) 13 completely oxidized to carbon diox1de and water a NET of how many ATP 5 IS formed? A 108 B 1 10.5 C l 16 A. malonyl CoA propionyl CoA acetyl CoA D. B-hydroxy-B-methyl butyryl CoA E. acetoacetyl CoA 9. Which of the following is a ketone body? A. laurjfate B-hydroxybutyrate B-hydroxy—B-methylglutaryl CoA D. pyruvate _ E. a—ketoglutarate 11. 12. 13. 15. Which of the following is NOT a substrate for the formation of carbamoyl phosphate in mitochondria? A. ammonia B. carbon dioxide C. ATP glutamate Which of the following is NOT involved in the methionine cycle in animal cells? A. S-Adenosyl methionine B. ATP C. S—Adenosyl homocysteine Ns-rnethyl tetrahydrofolate cysteine Which of the following does NOT directly filmish atoms in the synthesis of purine nucleotides? A. C02 B. glycine C. aSpartate D. glutamine ® serine phosphates? .. . 1 2 . 2.5 D. 3 E. 4 Which of the following is NOT a characteristic of tRNA? contains unusual bases has regions of Watson—Crick base pairing assumes an L—shape in three dimensions A. B. C. typically can recognize more than one codon @ bonds with an amino acid at the 5' end 17. 18. 19. 20. 1 DNA pol III 2. DNA pol I 3. DNA-dependent RNA polymerase 4 DNA ligase B 5:5 The operator region in E. coli has what fimetion? binds repressor '5 . binds RNA polymerase C. binds CAMP D. binds rho factor E binds small ribosomal subunit Shine-Dalgarno sequences have which function in prokaryotes? A. specify the initiation of DNA replication . establish which AUG will serve as the initiation codon . serve as a ribosomal binding site D serve to bind RNA polymerase E initiate the production of tRNA Post—translational processing of proteins in eukaryotes may typically involve which of the following? A. covalent addition of carbohydrate to some amino acid residues B. commitment of the nascent protein to the rough endoplasmic reticulum C. cleavage of a leader sequence from the amino terminus of the protein storage of protein product in vesicles E (All of the foregoing may occur.) In elongation of a growing polypeptide chain, an incoming amino acid-tRNA A. binds to the P site of the ribosome. B. recognizes a specific triplet on rRNA. C. forms a covalent bond with an initiation factor. . D. is hydrolyzed in an ATP-requiring reaction. ® recognizes a complementary region on mRNA.. Short Answer [Credit for each item is g‘ven in parentheses. 1 21. (6) Synthesis of palmitic acid (16:0) fi‘om mitochondrial acetyl CoA would involve how many of each of the following molecules? 7 malonyl CoA /5 ATP I t (NADPH + H") 22. (4) Briefly give two reasons why urea is suitable as an end~product of amino acid degradation in animal cells. sfl-[ué Xe 23. _ (3) There are three post-transcriptional modifications made to mRNA in eukaryotes. List those modifications. 24. - (8) Suppose that the mRNA shown below is used as a template for protein synthesis. Mel: 643 Sew fief‘ 5""? G ‘ ‘ UG G AA 5ppp5 GUCGAAUCC AU CA GACGflJCGGUGAGUE—AfiUA AGA 9th fig VA.) L35 Give the amino acid sequence that would be expected. (A copy. of the genetic code appears on the following page.) I 25. (4) What two major products result fiom the light reaction of photosynthesis, - and what are their roles in the overall process of photosynthesis. NRDPH - EMW‘“ 1063 26. When Hl‘cog' is added to a suspension of Chlorella, (a) (2) what is the first stable labeled co pound resul‘gggé' 3 Phasfhtzjfjaém (b) (2) what is the‘ substrate to which carbon dioxide adds], ' {‘IbLL/ABSQ #5 $15? GarretUGi‘isham, Biochemistry with a Human Focus Tabie 25.1 I Table 25.1 the Genetic We first _ Third Position Second ' Position {5'-end) Position (Shani) (DE-CC: mil-Gt: carbon: Gil-QC: _- * AUG signals translaii‘nn lnifiafion as well as coding for Mei residues. Third-Base Deannemq is Color-(Med mini Bass with 'i‘hird‘Basa Reiafionship Same meaning Number of Cations Third base irrelevant U, C, A, G 32 (8 families) Purines A or G 12 (6 pairs} Pyiimidines U or c 14 (7 pairs} Three out of four 11.6, A 3 (aux = lie) Unique definitions G only 2 (AUG # Met) (use = Tip) [:1 Unique definiii‘on A only 1 (UGA = Step) Harcourt, inc. items and derived items copyright © 2002 by Harcourt, Inc. 27. (5) When lactose is added to the culture medium of E. coli, induction of [3- galactosidase synthesis occurs. Breifly give the mechanism by which this occurs. W 28. (a) (3) Write a word equation for the reaction that occurs in an aminotransferase-catalyzed reaction between glutamate and ante' MUTP3r=7<KG+ _)m (b) (1) What co-factor is required? '57 04515354 / ? km (0) (2) Diagram a mechanism for the reaction. 7A ”-1 The End ...
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