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Sp07_Exam3_Key - Mutti te-Cheiee 3 011113 each 45 oinls...

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Unformatted text preview: Mutti te-Cheiee 3 011113 each 45 oinls total For each of the Following questions, encircle the letter corresponding to the BEST response. 1. Consider the following steps in [.5 oxidation ol'u fatty acid 1. introduction of a double bond ’2. oxidation of B —OH group to a. ['3 keto group 3. Iormation ol' CoA derivative 4 thiolytic cleavage 5 acylation of earnitine Which of the following gives the correct sequnce of events? A. 1-23-45 B. 5-32-44 (2. 4-5-3—1-2 D. 3—1—2-4-5 @ ”1-5— 1 24 2. When oleie acid (18:1) is completely oxidized to carbon dioxide and water a NET of how many ATP's is formed? a A. 104.5 4 ’4‘! (Wt r zz B. 106 6 53‘ _, 1?.- C. 110 p} ..» .r 5 Q) 113.5 <1 = C /—v—~"’ . 120 3’. Which ofthe following compounds is produced hy‘ the oxidation of C—odd. but not C-even Jetty acids? A malonyl CoA B. aeetyl CeA © methylmalonyl COA D. [l-hydt‘oxy-fl-metliyl butytyl CoA E. acetoacetyl CoA 4. Which of the following is a lee-tone. body? A. niyristoyl CoA B hydroxymethylglutan’l COA @ fi-h ydroxyhutyrate D. pyruvatc E. a-ketoglutarate A derivative of which vitamin is required for the conversion ol‘pyruvale to oxaloacetate‘? A thiamine B. riboflavin " niacin @ biotin ii. pyridoxine 1[Which of the following,1 is NU'l' a substrate for the formation of cathanmyl phosphate in tlie mitochondrion? A. ammonia B. carbon dioxide C ATP ([9 glutamine Which of the following is NOT involved in the methionine cycle in animal cells? A. S-Adenosyl methionine B. ATP ‘. S—Adenosyl homocystcine ® NS-N '0 methylene tetrahydrot‘olale F. homoeysteine Which of the Following does NOT directly furnish atoms in the synthesis ol‘ purine nucleotides? A [-301 glycine aspartate B. CL @ glutamate E. N 5- form yl tetrahydrot‘olate Formation of one peptide bond from an amino acid and an uncharged t-RNA requires the expenditure of how many high-energy phosphates? A. l B. 2 (I. 2.5. T). 3 CE} 4 [\J ll}. Which of the following is NOT a characteristic of most tRNA's'? A. contain unusual bases B. have regions of Watson—Crick base pairing C. assume an L-shape in three dimensions T). typically recognize more than one codon ® attach an amino acid at the 5’ end 11. Consider each of the following enzymes involved in DNA replication in E. calf: l . DNA pol Ill 2. DNA pol I 3. DNA—dependent RNA polymerase 4 DNA ligase Which of the l'ollowim rJives the correct SCL uence in which these enzvmes act“? LL: 1') —[ a A. 1.2—4.3 13. 2-1-3-4 e, 2—1-4—3 o. 3-2-4-1 ® 3—1-2-4 12. The operator region in the lac operon of E. coli has what function? ® binds i'epressor l3. binds RNA polymerase C. binds CAMP D. binds rho factor H. binds small ribosomal subunit lf-l. Shine—Dalgarno sequences have which function in prokaryotes‘? A. spec-ii}; the initiation ot‘DhA replication ® establish which AUG will serve as the initiation codon C. serve as a ribosomal binding site D. serve to bind RNA polymerase FL. initiate the production 01" [RNA 14. Post-translational processing of proteins in eukaryotes may typically involve which of the following? A. covalent addition of carbohydrate to some amino acid residues B. commitment of the nascent protein to the rough endoplasmic reticulum C. cleavage of‘a leader sequence from the amino terminus ol‘ the protein . delivery of the protein to lysosomcs ® (All of the loregoing may occur.) DJ 16 In elongation ofa growing polypeptide chain, an incoming amino acid-tRNA A. B. C. D @ l _ 0) (a) (b) (C) (d) (e) binds to the P site of the ribosome. recognizes a specific triplet on rRNA. forms a covalent bond with an initiation factor. is hydrolyzed in an ATP-requiring reaction. recognizes a complementary region on mRNA. Explain, define, or characterize briefly. biotin A cot'actor required for carboxylation reactions. transketolase A TPP dependent enzyme that catalyzes the tranfer of a ketol group to an aldose recepient. essential amino acid an amino acid that we cannot synthesize and must obtain from our diet phosphorylase Enzyme that facilitates the breakdown of glycogen into Glucose 1-phosphate and Glycogen (11—1) catabolite activator protein Protein complex that interacts Wlth CAMP and binds to the promoter region and facilitates the binding of RNA polymerase, intiti ati n g transcription of the lac operon. l7. l8. l9. (6) Synthesis of stenrie acid (18:0) liom mitochondrial acetyl CoA would m V olve how man y of each of the following molecules? 8' malonyl Cork 2 7 ATP MG (NADPH + Ht] (:2) Why is ammonia unsuitable as an end product of amino acid eatflholism in humans? ' ’fi/Sfiflfiw 5 (10) The template strand of DNA known to eneode the N-terminal region ol'an E. cell protein has the following nueleoticle sequence: 5'-GCCTA'l'ACTAGGACAATCATTACC-3' Give the amino acid sequence that would be expected. (A copy of the genetic code appears on the following page.) N—Formyl Met — Ile — Val — Leu - Val —C ‘JI Garreitt‘Grisham, Biochemistry with a Human Focus Table 25.1 Table 25.1 The Genetic Cede F irsi Third Pnsitiun Second Position {5'-end} Position (3'-end} U C .fi, G UUU m UCLE 8:3? UAU Ty: UGU Cys U UUC P59 ij-{JC Ear URI; Iy: UGC (lys C 31 lJUfi. LEI;- 126.11 35% HM 9111;: MBA Film-n fix UUG Lea 1383 Scar UAG Ste; UGG Tap G CUU’ L811 CCU Pm CAU His CGU Fug U CUE LEié CCC Pm (SAC His CEC- Arg C C CUé {tea CCA 9m (2AA SEE? CGA Arg fa CUG Lou (ECG Pro CAG Gin (EGG Aug G AUU 13:3 ACU Tm MU Asa AGU Ser U AUG lie AC6 Th! ARC Asr: ABC Ser C 9:. AUA rite . RCA Thr AAA ".ys ABA Arg A AUG Mare?“ AUG Th? __ AAG £335 MEG Arg G 6138 W GCU Ala GAL] Asp GGU Giy U GUS E’s-‘2} GCC Na SAC 3151:) GGL' 515: C {i GU11 Va: (56:! A56 GAR Gm GSA Gly 33 C} GUG V32 (ECG Ala GAG Gi-u GGG Gt}- .mmmmu translating maat m as weal as finding; for Me: rasidues. Third-Base Degeneracy Is Colorfinded Ihird Bases with Third-Base Helatinnship Same Meaning Number of Cndnns 'l'l'1ird base irraievant l}! {U311} 32?. {8' fwviiiex'} Pumas Ii. or G 12 {15 pairs; Pyrimédiaes U 3! C 13-1 ("f paws.) fi'iii‘it‘: Gui L1" 50!}! U. C. It 3 {_I\U?\’ -' We} Unique definitieris G [Jni'y 2 {AUG may} (UGG ----- Tm} A Only 1 {UGA Stop} Harcourt, Inc. items and derivad items copyright 3:) 2032 by Harcourt, Inc. 20. (8) Suppose than a random eta—polymer containing ”3/4 (3 and 1/4 C is used as a template for protein synthesis. Give the kinds and proportions ot‘amino acids in the resulting polypeptide. GGG 27/64 _ 61y GGC 9/64 Gly GCG 9/64 Ala GCC 3/64 Ala CGG 9/64 Arg CGC 3/64 Arg CCG 3/64 Pro CCC 1/64 Pro Se: 36/64 Gly 12/64 Ala 12/64 Arg 4/64 Pro 21. 45' Give the rule 01 C)LliL‘ 3’ -5 AM? 111 glycogen synthesis and degr dd Elliflfl D cflwfdcfiwfiés frflZk/MJ 12/ with “WP/451355;” $255 95%: 0133/4256: Mac/(£14 dew/‘95 3:9 3% Meg/“<95 I gag? kings S: 74 ’19 1170-” c1945” Jig}; @VLW‘S 2.5 22. (2') Give the ml 6 of 5 41M? 1 g unoneoqenesis; n6 é >1I$ 2 139:»: ’ 5 WP Add; byZPAWFhLTZfi—gf 23. (4) When l“'N-labeled ammonia is added to a suspension of liver cells, some glutamate labeled in its amino group can be subsequently isolated. Explain how this might occul W @DH PM )éfixjflm7éfl 041% + filjfl: my” NAM 1'” 24. (6) The first step in the degradation 0fvaline involves its c0111 ersiun to 0(- kctoisovaleric acid 111 an aminotransferase catalyzed ieaetien Skeleh a feasible mechanism for the 1111111011 including any other substrates and e0— 6:,/ 7am factors 25. (I) Fxplain briefly wl 1} {_I -edd fatty acids are somewhat r—IlLleogenie. firEakJéM} )7 M7 gfléé/il W 029 f} fierS EL ...
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