x2-spr09 - Name UIN MCB 450 Exam II This exam consists of...

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Name: __________________________________ UIN: ______________________ MCB 450 Exam II March 19, 2009 This exam consists of 18 multiple choice questions and 12 short answer questions. There are 11 pages (front and back) NOT including the cover page, a page with graph paper, and a blank back page. 23. PRINT your name and UIN at the top of this page . 24. PRINT your name at the top of each page of the exam (both front and back) Note: Proctors cannot answer questions or make clarifications. Please read each question carefully and answer to the best of your ability. When you have completed the exam, hand it in to a proctor and show them your Student ID card. Good luck! 1
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Name: __________________________________ Multiple-Choice, 2 points each, 36 points total For each of the following questions, encircle the letter corresponding to the BEST response. 1. Which of the following is NOT an assumption made in the derivation of the Michaselis-Menten equation? A. The reaction proceeds with a large, negative change in free energy. B. The rate of formation of ES is balanced by the rate of its destruction, either to E + S or to P + E. C. The k -2 step can be ignored. D. Only one substrate is considered. E. (All of the above are assumptions usually made). 2. Which of the following correctly defines K M ? A. It is equal to the quantity k 1 /(k -1 + k 2 ) B. It is the dissociation constant for the enzyme-substrate complex. C. It is equal to V max /2. D. It is the substrate concentration giving half-maximal velocity. E. It is equal to the quantity k -1 /(k 1 + k 2 ) 3. Diisopropylfluorophosphate (DIFP) inactivates trypsin, chymotrypsin, acetylcholinesterase, and many other enzymes. What best explains the basis for this effect? A. It serves as a noncompetitive inhibitor of those enzymes. B. It forms a covalent bond with the –OH group of Ser. C. It binds to the active site of those enzymes, preventing binding of substrate. D. It binds to the Asp residue in the catalytic triad. E. It interferes with proton exchange by the sidechain of Lys. 4. Which statement about a hemoglobin molecule is INCORRECT? A.
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x2-spr09 - Name UIN MCB 450 Exam II This exam consists of...

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