x3-key-F08

x3-key-F08 - 20. (1 pt for each codon) 27/64 GGG Gly 9/64...

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MCB 450 Fall 2008 Examination Three – Answer Key 1. E 2. D 3. C 4. C 5. D 6. D 7. D 8. D 9. E 10. E 11. E 12. A 13. B 14. E 15. E 16. 1. (1 pt) Vitamin with many derivatives, each transferring a single carbon atom at different oxidation levels. (1 pt) Involved in purine nucleotide biosynthesis and methionine cycle. 2. Transfers the ketol group to acceptor aldehyde (1 pt). Requires TPP (1 pt). 3. One we cannot synthesize (1 pt) and which must be supplied in our diet (1 pt). 4. Enzyme degrading glycogen (1 pt) in liver, kidney, and muscle (1 pt). 5. Involved in lactose operon of E. coli. Binds to cAMP. (1 pt) CAP-cAMP facilitates binding of RNA polymerase to promoter (1 pt). 17. 8 malonyl CoA; 17 ATP; 16 NADPH + H+ (2 pts each) 18. 2 lactate + 2H + + 4ATP + 2GTP + 6H 2 O -> Glucose + 4ADP + 2GDP + 6Pi (correct components, 2pts; correct ratio, 2pts). 19. Met-Ile-Val-Leu-Val (3 pts if mRNA’s sequence is correct; 2 pts for choosing the correct AUG site and 5’-3’ direction; 3 pts for correct peptide sequence)
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Unformatted text preview: 20. (1 pt for each codon) 27/64 GGG Gly 9/64 GGU Gly 9/64 GUG Val 3/64 GUU Val 9/64 UGG Trp 3/64 UGU Cys 3/64 UUG Leu 1/64 UUU Phe 21. It activates protein kinase A which phosphorylates phosphorylase kinase and glycogen synthase (2 pts). Phosphorylase kinase in turn phosphorylates phosphorylase making it more active (1 pt). The phosphorylated form of glycogen synthase is less active (1 pt). 22. (1 pt) Binds to CAP; (1 pt) CAP-cAMP facilitates binding of RNA polymerase to promoter. 23. (2 pts) NH3 + KG interact to give a labeled amino group of Glu; enzyme = glutamate dehydrogenase; (2 pts) Then the following aminotransferase reaction gives labeled Ala: Glu + pyruvate = Ala + KG 24. (3 pts) Val + Enz-Pyr-CHO = Enz-Pyr-CH 2 NH 2 + -KIV (3 pts) Enz-Pyr-CH 2 NH 2 + KG = Enz-Pyr-CHO + Glu 25. Terminal3-carbon fragment is prop CoA (1 pt) methymalonyl CoA succinyl CoA (1 pt) OAA (1pt) Glucose in gluconeogenesis....
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x3-key-F08 - 20. (1 pt for each codon) 27/64 GGG Gly 9/64...

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