Biotech - place DNA fragments in well in porous gel w...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Biotech 16.1 DNA molecules and Analysis restriction enzyme cleave DNA @ a specific site -bacterial cells use ‘em to defend against invading pahges - protect own geneome through methlization -called restrictive digestion - recognition sequences or restriction sites cleaved segments of DNA has a specific sequence of bases -recognized through protein DNA interaction - can be used to genetically cut off segments Gel electrophoresis -after being cut w/ restriction enzyme, fragments must be separated -need to separate different sizes of fragments
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: - place DNA fragments in well in porous gel w/ electric field (- and +)-DNA is (-) cuz of phosophate-after fixed time, e- turned off -visulisatoin through florescent dye-size of fragments-presence of specific DNA sequences-single stranded DNA probe-DNA denatured and transferred to nylon filter for a southern blot-exposed to single stranded DNA probe (sequence matched to fragment of interest)-labeled radioisotope of probe-unbounded probe stays in solution...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online