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DNA and Heredity - 11.1 Evidence that DNA holds genetic...

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11.1 Evidence that DNA holds genetic Material Fredrick Griffith’s Experiment on 2 types of pneumococcie (® rough and (s) smooth) - Fredrick Griffith, English -lethal (s) vs. nonlethal ® strains - inoculate lethal (s) w/ heat, add nonlethal ® and becomes lethal - demonstrates that there is a physical genetic material present Oswald Avery’s Experiment on pneumococci - Rockefeller University - inoculated strain of (s) strain, separated into 3 categories, added DNase, RNase, and Protienase - both (s) and ® strains grew in RNase and Protienase, only ® grew in DNase - demonstrations that DNA is the genetic material Hershey Chase Experiment with Radioisotope Phages - T2 phages infect bacterial cells - label 2 T2 phages colonies, one with S 35 (tracks proteins) and other with P 32 (track DNA) - aggregate cells/phage after insertion of DNA, phage protein coat left in solution - centrifuge sample, e. coli pellet reads for P 32 and solution reads S 35 - demonstrates that viruses insert their DNA, not their proteins; that viral
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