Ch. 11 - Ch 11 DNA DNA genetic material found in nucleus...

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Ch. 11- DNA DNA: genetic material found in nucleus stored on chromosomes double helix 4 possible bases for 4 nucleotides Dyes/stains- somatic cells had twice as much DNA as the gametes. (important for reproduction) Every species will have a specific amount of DNA. Experiment: looking for vaccine against ammonia R strain. Could be injected in a mouse- mouse lives, S strain- the mouse will die. Heat killed bacteria that killed the mice! Then mixed heat killed with dead bacteria, S bacteria with R strain (which is alive)- the mouse dies with a live S in the heart. The dead S strain transformed live R into a killer bacteria- transforming principle! Oswald Avery’s 2 nd experiment: identified the transforming principle by process of elimination. S dead= virulent R alive= non-virulent They mix them together and looked at RNA, protein, RNA. Treat each other with agents RNase-broke down RNA-still transformed. Protease- broke down protein- still transformed.
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DNase- destroyed the transforming ability of the bacteria-if bacteria had no DNA- then no transformation. DNA agent responsible & transformation principle.
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