Quiz 2 answers

Quiz 2 answers - Cell Biology BioS 222 Fall 2009 Quiz 2...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Cell Biology BioS 222 Fall 2009 Quiz 2 NAME:_________________________________________ TOTAL 22 points 1. You wish to produce a human enzyme, protein A, by introducing its gene into bacteria. The genetically engineered bacteria make large amounts of protein A, but it is in the form of an insoluble aggregate with no enzymatic activity. Which of the following procedures might help you to obtain soluble, enzymatically active protein? Explain your reasoning. (6 points) A. Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea. B. Treat the insoluble aggregate with a protease. C. Make the bacteria overproduce chaperone proteins in addition to protein A. ANSWER: Choices A and C are worth trying. Some proteins require molecular chaperones if they are to fold properly within the environment of the cell. In the absence of chaperones, a partly folded polypeptide chain has exposed amino acids that can form noncovalent bonds with other regions of the protein itself and with other proteins, thus causing nonspecific aggregation of proteins. A.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Quiz 2 answers - Cell Biology BioS 222 Fall 2009 Quiz 2...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online