Lecture 5 - Patch Clamping can record current passing...

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Patch Clamping can record current passing through An individual ion channel
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Inventors of the patch clamp Erwin Neher (1944- Bert Sakmann (1942- Nobel prize 1991
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Record from a patch many times. Average the responses. Compare with the current recorded from the whole cell. The G-V curve is a curve of the probability of channel opening vs voltage.
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g K = g K(max) n 4 g Na = g Na(max) m 3 h
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Relationship between gating current and ionic current: estimates of ~3-4 charges/channel subunit.
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Gating current is greater and faster at more depolarized voltages.
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Ion channels and disease Hyperkalemic periodic paralysis (HYPP)--muscle. Long QT syndrome (LQT)--cardiac. Lambert-Eaton syndrome (LES)--motor neuron.
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http://www.youtube.com/watch? v=j5kKoBOfPJk http://www.youtube.com/watch? v=zx7kAJD0faA http://www.youtube.com/watch? v=P4yNEKNHk8E&feature=related
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Ion channels and disease Hyperkalemic periodic paralysis (HYPP)--muscle. Hyperkalemia: blood K + levels; following exercise or eating K + -rich food. Periodic paralysis: a paralysis that occurs periodically. Symptoms: muscle stiffness (myotonia), to paralysis of shoulder, hips, or limbs. Episodes last a few hours. Affects humans and horses (descendents of “Impressive”).
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http://www.tufts.edu/vet/vet_common/images/sports/hypp/Fgure1.jpg
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This note was uploaded on 09/17/2009 for the course BIO 365R taught by Professor Draper during the Spring '08 term at University of Texas at Austin.

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Lecture 5 - Patch Clamping can record current passing...

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