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BISC_320_Exam_3_2004

BISC_320_Exam_3_2004 - Last Name(print m First Name(print 5...

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Unformatted text preview: Last Name(print) m _ First Name(print) - 5%} Lab Section (time and day) “Ada! @) 900 TAs Mmad 9’ I?” g: {7 BISC 320 Midterm Exam 3 - 11/10/04 Total Score (out of 100 pts) 93 Please note: if you use pencil or whiteout, you will have NO chance for a re-grade. / l. (10 pts) How is sigma 70 protein utilized in initiation of transcription in prokaryotes? nga '10 9mm mm m ’W/ afiachmwf 0f RNApM/gwmso 1;» mRNA. 975ch 10 mm was 1h 1m ,3; a -10 by PWM‘M gnu/tome. W W M omwwmw was to we grew 70 9mm. «39 "0 +4 T emu sign/mo LbNZA/ps mm pmmmw fwd WW yawn/cw wqmw 2. (10 pts) How is the carboxy terminal domain (CTD) of the large subunit of eucaryotic RNA polymerase II utilized during transcription? 010 cmm'ns W ropeami mamas of 7 amino nerds. i’r wing 2nd 06mm 0? gum/10W VII/"WW0 WWI/'0 6W! \3 mum/awhiw . up cal/ms V 713 W W sew gm mllNAmha. my fish mm aamswm, 3 WWW yMUsPMy ’rcdym/mw ma mum/WM mo 00) 17M Mvmg W wtw/ISWA fw making w 'm» The up TM is M90 (WWW MW in by, Wm WM fW W3” 0% mWA SW1d. M M V a?” Name(print) - First Name(print)-____ . (lOpts) Briefly, how (_by what mechanism) do lariat structures form in splicing ~pre—mRNA? -. UAW“ WWWS favm by mousing) m S‘e 2\ WW4 Wm W S‘ spr am M; W m’mm omd mo 2' 0H 0} w Wtw. .4" \11 \n P MM WWW? gm. Wm M? Wale -w 9‘ swim «to _ Jvo kiwkvamtaun mm fivmgmmp amt/W by WPwmww PawnL mam VWWD. MBgRNP WW owes W 3‘ WW symw gm, a 2‘-§' mego 4. (10 pts) Of what value is an SR protein that binds to an ESE sequence within an exon in a pre—mRNA strand? 89 Mom 'bmdmg «2:» am 696 madam wwpwswm of w my} , - 9% 3mm: fqv (mm 9W cm (ant/WW. SR pumm- vmd 17» W2 ESE (3 WWWSIAMMM and wmltmg ma gpuwowww ywwm “mi-M) WWWW scqwmw, mum m M «g Wm. «SR pwwm aw WWW/MN alum/w mm If an mam/mm Is (WW/SM (W “M W ,J’yvName (print) ‘ First Name(print) - 5. (10 pts) In post—transcription editing of mRNA, how can deamination Change a sense codon into a translation stop codon? m Mammmomm, cytosww (mwmaga mam (7M own/w gun/1p ) Mm 05%me Wm, M WW 1,» a mom Wocu . \V\ W MPNR mammm (Mi/V1 MW reads UAAmWh 01011 as 0\ 3M) Mm 'W WNW/m. 2 Wm "WA 3‘ A CAR 3' Q9 (ammo (Leahimm 3‘ M 31 WW 6. (10 pts) Briefly, how is transcription termination, with position specificity, accomplished in procaryotes and eucaryotes? (Do not bother to mention any protein factors involved . ) WWW Wmma’wm achwcdb mo «weanm m we \mdapmam’r. In m W‘del, W) VNP‘ muém Mum/mm WWW mm @551ng 38%wa Wm WM MMSQAW mm mMWw sequmw W \MM amMm/VKI mm Vamm‘dewg mm m man 6W, mu 51mg mm A Eamm 0W Wait pm mp.W, 0\Jr W162 6M 1% W WW \0 mm W0 aw - Wk?” mama OHM/WW A-T wake PM? W W AS VNAWW “5 w wmdxyvzgaWMWTM/V A W‘ W W mmw am mu: (M WW) Mm Ic. mmfiw W mm: mm mm WW ama WIRNA IS puma OFF. M WM 1 mug Wm Wm WM and MP MM ap _ , Rh; WW ‘4'. ‘5 it: “PM" DNA WPMW MN MS WWWIAl: ;.NA WW6 ye 3mm, [gamma mwvwmawm 9+0 W MW . M WW WWW 1 WW Sequel/lac MN MA. \0990 hp Law fiw mKLNA 5mm \‘5 out flame (print) a First Name (print)_______ fl. (10 pts) (a) What is meant by the term ORF (open reading Q /frame) as it is used in protein synthesis? (b) How many . translational reading frames are possible on the same mRNA strand? Oh‘WW MWA I} @ ORF ‘SWOW WWW WNW) WWW m We W11 WV WWW HMS“. \QAthWS W and mm W1 “ BR? WWW Wm MOW/‘8 (M W A vmfh 111 WWW); 8. (10 pts) In prokaryotes, how does translation initiate? to M3 t .' , 1 a n 7:: 1 :‘(I , ‘ fiv'r" [jakrmtfi‘r-fi a . u (If /‘I‘;a’i; W 30:, b m om-‘mung an RNA WWI/1C0 WAN mlbsr TV“; \bs HZNA WW W20 mm mm 8" meégW $6qu mom. WAS, 30; 9!?qu IS anmow wen/A ~§ : W‘WWWWISAVQ’CA MW, 0111465141 AW $ 4. / 4 4 A) WA MW _ m b g: 3 if .9 E. E 9.5 f? {E 2 PM“ "WWW WA \onX m A cm to va‘r “W A GU» 6? UNI/305 wwm M mWMV? CaTP was @1111 30: 91 «11mm (m) a WOT/WA “W Wfiuw $1111thme MWA vm\\\p\v\A£T/%. WW TAWBSU’CJpw/VW mg 305 WWVM firm/w (1mg ma «9 v: law; 305 sum/1H Scams 0mg 1 gym Mm . $515qu . WW2 WWW’M MWOIMS, 1 § m \Fl M2036. _ Name(print) 1 First Name(print) -' . (10 pts) What is the purpose of the A, P, and E sites on ribosomes? A g‘H’C W “WW/1b “Cali WA cam/31mg W W “WWW aa'd VIVIGLC . ”(7e W13 gm. v - P 9% WW5 WA mm \m M3 cm. 9m {w WA erh w, wawg pol/5mm Mam. E aw: aw vWM W M WNA Wt’r um to \vmaWr W PSHU?» mwes, Max/VIM W ammo- a .1 mm a, wow W. MhMLWW mm av: 01 vww mam mm® cam W‘V‘xw WWW” M W 00M“- W\ W A No HMS WW pflwgploghflmam M PataWJ ' WWW Mm .WW/I yaw nah/A W M 0mm stma avor b» A_1 $33312 mm Ame mm was to pch. 10. (10 pts) (a) How do stop codons manage to stop protein synthesis? (b) How do ribosomes then release from mRNA? /[email protected] pwwvu m w mommy/W WSMp ngghp 060W“ are W'Ww WSW ’MM almm‘wwofw . m OWN) (A .Wfivvéflvw WWW WA \MWH M/fisl’w - mawFaMwym mi WWW MM. PWWW: law mm mm and 1m RF2= mas MM and MA WWW ml'- was an W5 9m? Wm: (umm, um . /= sun/mam wauwmm the w m and m ‘ 92% WOW Md vwwm (mafia, mum) mm W AW «em W a m 0’; mm 305 omd we; guvuvi\T9.WA/"WW WW 23W“ MSWVWW/ W W WWW, wwwoxuww alsowwcs W W! WW- ...
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