SAMPLE QUIZ QUESTIONS 150 (L) DNA FINGER

SAMPLE QUIZ QUESTIONS 150 (L) DNA FINGER - b. DNA...

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BIOLOGY 150, SAMPLE QUIZ QUESTIONS, SECTION L: DNA FINGERPRINTING & GEL ELECTROPHORESIS The following gels were run on samples of blood collected from a crime scene (labeled CS on the gels) and blood collected from three suspects (labeled F, G, and H respectively on the gels). All the samples were cut with four different restriction enzymes (numbered 1, 2, 3, and 4). Answer questions 1-2 based on these gels. Restriction enzyme 1 Restriction enzyme 2 Restriction enzyme 3 Restriction enzyme 4 1) According to restriction enzyme 2, which of the three suspect is/are most likely connected to the crime scene? F G H F & G G & H F & H F, G & H none 2) According to the combined results of all the gels which of the three suspect is/are most likely connected to the F G H F & G G & H F & H F, G & H none 3) Which of the following is INCORRECT regarding gel electrophoresis? a. Gels must be stained before DNA is visible.
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Unformatted text preview: b. DNA restriction fragments move based on charge. d. DNA restriction fragments separate because of size differences. d. DNA restriction fragments separate because the have adenine. 4) When preparing a DNA gel for gel electrophoresis, which of the following is loaded into the wells of the gel? a. samples of mRNA b. samples of uracil c. samples of HIV d. samples of DNA 5) Why is it important to use more than one restriction enzyme in gel electrophoresis? 6) Describe one use of the technology of DNA fingerprinting other than criminal investigations and paternity suits. 7) Which of the following is used to load DNA samples into gel wells? a. forklift b. beaker c. syringe d. micropipette 8) What about the DNA causes the fragments to separate out on the gel? 9) Briefly describe restriction enzymes and how they are used in gel electrophoresis. 10) Briefly describe PCR and how it is used in gel electrophoresis. CS F G H CS F G H CS F G H CS F G H...
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This note was uploaded on 09/18/2009 for the course BIOL 5200 taught by Professor Dr.juliecampbell during the Fall '09 term at Kansas.

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