Lecture 34 Bacteria slides

Lecture 34 Bacteria slides - Bacterial Genetics Part I How...

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Bacterial Genetics Part I. How bacteria exchange genes 6th: 331-332; 340-344 7th: 337-338; 346-350 Part II. Regulation of gene expression 6th: 347-349 7th: 352-354
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3 ways bacteria can acquire new DNA 1.Transformation : uptake of free DNA 2.Transduction : bacteriophages carry DNA between cells 3.Conjugation : “bacterial sex”; transfer of chromosomal DNA between bacteria
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Sizes of phage, bacterium & animal cell
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Phage life cycle 1.Phage injects DNA genome into bacterium 2.Bacterial DNA is chopped up. 3.New viral proteins & viral DNA are made by bacterial enzymes; they self-assemble to make new virus particles.
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Transduction: viral proteins accidentally assemble with a bacterial DNA fragment instead of the viral genome. Phage with bacterial DNA is released from the cell. bacterial DNA fragments
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This phage injects the DNA fragment into ano- ther bacterium. The fragment recombines with the bacterial chromo- some; breaking/rejoining at equivalent positions. Injected A+ form of gene replaces original A- form. recombinant cell
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Conjugation: temporary joining of 2 bacteria by sex pili extending from 1 cell. Sex pilus: hollow tube; DNA moves through it between cells.
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