Lecture 35 Euk Gene Reg slides

Lecture 35 Euk Gene Reg slides - Control of Eukaryotic Gene...

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Control of Eukaryotic Gene Expression ! 6th: 354-356; 362-367 (top) 7th: 316 & Fig. 17.8; 359-367
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Only a subset of genes is expressed at any time. Different cells need different proteins at different times. Usually; the amount of a protein in a cell is set by regulating the rate of transcription of its gene . If an mRNA is made, it’s usually automatically translated into protein.
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Review: genes are in operons in bacteria promoter operator RNA pol. Genes in operon Repressor protein can bind operator to block RNA polymerase.
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Eukaryotes have NO: -operons or operator -repressor that physically blocks RNA polymerase Rarely have regulatory proteins that bind small repressor in bacteria.) Eukaryotic RNA pol. doesn’t even bind the promoter by itself!
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Eukaryotic promoter usually has TATA box ~25 bp (base pairs) upstream of the start site of transcription Fig 17.8, 7th ed
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Several proteins called transcription factors first bind to DNA. One of these binds the TATA box. TATA box
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RNA pol. can bind the promoter once the transcription factors have bound. RNA pol. + these factors form the trans- cription initiation complex
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These are general trans- cription factors; they act in transcription of all genes. Some transcription factors bind to DNA; some bind to other transcription factors.
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complex doesn’t bind the promoter well. By itself; it gives very low transcription. Additional “
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Lecture 35 Euk Gene Reg slides - Control of Eukaryotic Gene...

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