suny notes 2 - Microscopy How to see the little ones How...

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Unformatted text preview: Microscopy How to see the little ones How small are they? Viruses - 50-100 nanometers Bacteria - 1-5 micrometers Eukaryotic cells - 10-100 micrometers Two major aspects of microscopy: MagniFcation and Resolution MagniFcation - an ability to make objects appear larger, expressed as a number combined with X (times). Example: A microscope magniFes a specimen by 100X. (This means the object looks 100 times as large as it really is). Two major aspects of microscopy: MagniFcation and Resolution Resolution - the ability to show detail, to see magniFed objects clearly. OfFcially deFned as the ability of an optical system to distinguish between two adjacent points. Resolving power has to do with the wavelength of light employed for viewing, as well as other technical considerations. Two major aspects of microscopy: MagniFcation and Resolution A good illustration of the relationship: Even if a light microscope could be designed to magnify e.g. 10,000X, its resolving power COULD NOT be increased (due to the wavelength of visible light), and the enlarged image would be big but fuzzy. The Compound Light Microscope (Brightfeld Microscopy) Two lenses: the ocular...
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suny notes 2 - Microscopy How to see the little ones How...

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