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Chapter 14 - Chapter 14 From Gene to Phenotype Dominance...

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Chapter 14 From Gene to Phenotype Dominance, epistasis, gene interaction •Genetic and environmental contributions interact to determine phenotype. •Mutations in same gene usually result in mutant phenotype when present together. •Mutations in different genes complement. •Dominance comes in several types. •Most characters are determined by sets of genes that interact with the environment. •Modified genetic ratios reveal gene interactions, including epistasis. Complementation test •Diagnostic test for a llelism •Mutants are crossed to bring two recessive mutations together in heterozygous form if phenotype is mutant, mutations are in same gene •they fail to complement •both have loss of function if phenotype is wild-type, mutations are in different gene •each mutant contributes normal gene at different locus •complementation due to interaction of different proteins •Heterokaryons used in haploid organisms Interactions between alleles •Dominant versus recessive
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